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Literature summary for 3.5.1.B15 extracted from
- Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics (2014), Biochemistry, 79, 54-61.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Acidilobus saccharovorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Acidilobus saccharovorans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme contains Fe2+ in the active site | Acidilobus saccharovorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46400 | - |
- |
Acidilobus saccharovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidilobus saccharovorans | D9Q1R5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyrazinamide + H2O | the enzyme also catalyzes the hydrolysis of nicotinamide | Acidilobus saccharovorans | pyrazinoic acid + NH3 | - |
? | |
| pyrazinamide + H2O | the enzyme also catalyzes the hydrolysis of nicotinamide | Acidilobus saccharovorans DSM 16705 | pyrazinoic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Acidilobus saccharovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ASAC_0847 | locus name | Acidilobus saccharovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Acidilobus saccharovorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
t1/2: 180 min | Acidilobus saccharovorans |
| 80 | - |
t1/2: 35 min | Acidilobus saccharovorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 135.7 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Acidilobus saccharovorans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 331 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
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