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Literature summary for 3.5.1.B15 extracted from

  • Fyfe, P.; Rao, V.; Zemla, A.; Cameron, S.; Hunter, W.
    Specificity and mechanism of Acinetobacter baumanii nicotinamidase: Implications for activation of the front-line tuberculosis drug pyrazinamide (2009), Angew. Chem. Int. Ed. Engl., 48, 9176-9179.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.B15

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates Acinetobacter baumannii
Mn2+ activates Acinetobacter baumannii
additional information PncA is a divalent cation-dependent enzyme Acinetobacter baumannii
Zn2+ activates, best cation, the active site Cys159 is located at the N-terminus of a6 at one side of the active site with the Zn2+ ion positioned on the other side. The metal ion is bound tightly in the AbPncA active site Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 gel filtratrion and analytical ultracentrifugation Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyrazinamide + H2O Acinetobacter baumannii a nicotinamide prodrug. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid pyrazinoic acid + NH3 a bacteriostatic drug ?

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyrazinamide + H2O a nicotinamide prodrug. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid Acinetobacter baumannii pyrazinoic acid + NH3 a bacteriostatic drug ?

Subunits

Subunits Comment Organism
dimer
-
 Acinetobacter baumannii

Synonyms

Synonyms Comment Organism
PncA
-
 Acinetobacter baumannii
pyrazinamidase
-
 Acinetobacter baumannii

General Information

General Information Comment Organism
metabolism in Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid Acinetobacter baumannii
physiological function the reaction product pyrazinoic acid inhibits Mycobacterium tuberculosis type I fatty acid synthase, represses mycolic acid biosynthesis, and appears to affect membrane energetics and acidification of the cytoplasm Acinetobacter baumannii