Protein Variants | Comment | Organism |
---|---|---|
K142A | mutation abolishes the property of FAAH to degrade amides and esters with equivalent catalytic efficiencies, generating a catalytically compromised enzyme that hydrolyzes esters more than 500fold faster than amides. Mutant enzyme shows an altered pH-rate profile | Rattus norvegicus |
K142E | mutant enzyme displays severely diminished catalytic activity, but one that now maintains ability of FAAG to react with amides and esters at competitive rates. Mutant enzyme shows an altered pH-rate profile | Rattus norvegicus |
K142Q | mutation abolishes the property of FAAH to degrade amides and esters with equivalent catalytic efficiencies, generating a catalytically compromised enzyme that hydrolyzes esters more than 500fold faster than amides. Mutant enzyme shows an altered pH-rate profile | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
oleoyl methyl amide | pH 9.0, wild-type enzyme | Rattus norvegicus | |
0.012 | - |
oleoyl p-nitroanilide | pH 9.0, mutant enzyme K142A | Rattus norvegicus | |
0.02 | - |
oleamide | pH 9.0, mutant enzyme K142A | Rattus norvegicus | |
0.021 | - |
oleoyl p-nitroanilide | pH 9.0, wild-type enzyme | Rattus norvegicus | |
0.022 | - |
oleoyl methyl ester | pH 9.0, wild-type enzyme | Rattus norvegicus | |
0.032 | - |
oleoyl methyl ester | pH 9.0, mutant enzyme K142E | Rattus norvegicus | |
0.037 | - |
oleamide | pH 9.0, wild-type enzyme | Rattus norvegicus | |
0.041 | - |
oleoyl methyl amide | pH 9.0, mutant enzyme K142A | Rattus norvegicus | |
0.063 | - |
oleoyl methyl ester | pH 9.0, mutant enzyme K142A | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleamide + H2O | - |
Rattus norvegicus | oleic acid + NH3 | - |
? | |
oleoyl methyl amide + H2O | - |
Rattus norvegicus | ? | - |
? | |
oleoyl methyl ester + H2O | - |
Rattus norvegicus | ? | - |
? | |
oleoyl p-nitroanilide + H2O | - |
Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAAH | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
oleoyl methyl amide | pH 9.0, mutant enzyme K142A | Rattus norvegicus | |
0.0002 | - |
oleoyl methyl ester | pH 9.0, mutant enzyme K142E | Rattus norvegicus | |
0.00026 | - |
oleamide | pH 9.0, mutant enzyme K142A | Rattus norvegicus | |
0.00031 | - |
oleoyl methyl amide | pH 9.0, mutant enzyme K142E | Rattus norvegicus | |
0.00052 | - |
oleoyl p-nitroanilide | pH 9.0, mutant enzyme K142E | Rattus norvegicus | |
0.00064 | - |
oleamide | pH 9.0, mutant enzyme K142E | Rattus norvegicus | |
0.012 | - |
oleoyl methyl ester | pH 9.0, mutant enzyme K142A | Rattus norvegicus | |
0.15 | - |
oleoyl p-nitroanilide | pH 9.0, mutant enzyme K142A | Rattus norvegicus | |
0.17 | - |
oleoyl methyl ester | pH 9.0, wild-type enzyme | Rattus norvegicus | |
1.9 | - |
oleoyl methyl amide | pH 9.0, wild-type enzyme | Rattus norvegicus | |
2.8 | - |
oleoyl p-nitroanilide | pH 9.0, wild-type enzyme | Rattus norvegicus | |
9 | - |
oleamide | pH 9.0, wild-type enzyme | Rattus norvegicus |