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Literature summary for 3.5.1.99 extracted from

  • Patricelli, M.P.; Cravatt, B.F.
    Fatty acid amide hydrolase competitively degrades bioactive amides and esters through a nonconventional catalytic mechanism (1999), Biochemistry, 38, 14125-14130.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
K142A mutation abolishes the property of FAAH to degrade amides and esters with equivalent catalytic efficiencies, generating a catalytically compromised enzyme that hydrolyzes esters more than 500fold faster than amides. Mutant enzyme shows an altered pH-rate profile Rattus norvegicus
K142E mutant enzyme displays severely diminished catalytic activity, but one that now maintains ability of FAAG to react with amides and esters at competitive rates. Mutant enzyme shows an altered pH-rate profile Rattus norvegicus
K142Q mutation abolishes the property of FAAH to degrade amides and esters with equivalent catalytic efficiencies, generating a catalytically compromised enzyme that hydrolyzes esters more than 500fold faster than amides. Mutant enzyme shows an altered pH-rate profile Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
oleoyl methyl amide pH 9.0, wild-type enzyme Rattus norvegicus
0.012
-
oleoyl p-nitroanilide pH 9.0, mutant enzyme K142A Rattus norvegicus
0.02
-
oleamide pH 9.0, mutant enzyme K142A Rattus norvegicus
0.021
-
oleoyl p-nitroanilide pH 9.0, wild-type enzyme Rattus norvegicus
0.022
-
oleoyl methyl ester pH 9.0, wild-type enzyme Rattus norvegicus
0.032
-
oleoyl methyl ester pH 9.0, mutant enzyme K142E Rattus norvegicus
0.037
-
oleamide pH 9.0, wild-type enzyme Rattus norvegicus
0.041
-
oleoyl methyl amide pH 9.0, mutant enzyme K142A Rattus norvegicus
0.063
-
oleoyl methyl ester pH 9.0, mutant enzyme K142A Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oleamide + H2O
-
Rattus norvegicus oleic acid + NH3
-
?
oleoyl methyl amide + H2O
-
Rattus norvegicus ?
-
?
oleoyl methyl ester + H2O
-
Rattus norvegicus ?
-
?
oleoyl p-nitroanilide + H2O
-
Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
FAAH
-
Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0001
-
oleoyl methyl amide pH 9.0, mutant enzyme K142A Rattus norvegicus
0.0002
-
oleoyl methyl ester pH 9.0, mutant enzyme K142E Rattus norvegicus
0.00026
-
oleamide pH 9.0, mutant enzyme K142A Rattus norvegicus
0.00031
-
oleoyl methyl amide pH 9.0, mutant enzyme K142E Rattus norvegicus
0.00052
-
oleoyl p-nitroanilide pH 9.0, mutant enzyme K142E Rattus norvegicus
0.00064
-
oleamide pH 9.0, mutant enzyme K142E Rattus norvegicus
0.012
-
oleoyl methyl ester pH 9.0, mutant enzyme K142A Rattus norvegicus
0.15
-
oleoyl p-nitroanilide pH 9.0, mutant enzyme K142A Rattus norvegicus
0.17
-
oleoyl methyl ester pH 9.0, wild-type enzyme Rattus norvegicus
1.9
-
oleoyl methyl amide pH 9.0, wild-type enzyme Rattus norvegicus
2.8
-
oleoyl p-nitroanilide pH 9.0, wild-type enzyme Rattus norvegicus
9
-
oleamide pH 9.0, wild-type enzyme Rattus norvegicus