Application | Comment | Organism |
---|---|---|
medicine | HDAC2 is nitrated under nitrative/oxidative stress and in the peripheral lung tissues of smokers and patients with chronic obstructive pulmonary disease | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y146A | mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation | Homo sapiens |
Y146A | in presence of peroxynitrite, activity is reduced by 32%. In presence of 3-morpholinosydonomine, activity is reduced similar to wild-type | Homo sapiens |
Y153A | mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation | Homo sapiens |
Y153A | in presence of 3-morpholinosydonomine, activity is reduced similar to wild-type. In presence of peroxynitrite, activity is abolished | Homo sapiens |
Y253A | mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation | Homo sapiens |
Y253A | Y253 is at least partly responsible for the proteasomal degradation of HDAC2 under nitrative stress. Activity is not reduced in presence of 3-morpholinosydonomine | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | nitration of distinct tyrosine residues; nitrative/oxidative stress reduce HDAC2 expression via nitration of distinct tyrosine residues. Peroxynitrite, hydrogen peroxide and cigarette smoke-conditioned medium reduce HDAC2 expression in A549 epithelial cells in vitro. This reduction is due to increased proteasomal degradation following ubiquitination rather than reduction of mRNA expression or stability | Homo sapiens | |
peroxynitrite | activity of wild-type enzyme is reduced to 38% in presence of peroxynitrite. Activities of mutants Y153A and Y253A are 233 completely abolished in the presence of peroxynitrite. Mutant Y146A shows 32% reduction in activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
determined by SDS-PAGE and Western Blot analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q92769 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
lung | - |
Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
HDAC2 | - |
Homo sapiens |
histone deacetylase 2 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | nitrative/oxidative stress reduce HDAC2 expression via nitration of distinct tyrosine residues. Peroxynitrite, hydrogen peroxide and cigarette smoke-conditioned medium reduce HDAC2 expression in A549 epithelial cells in vitro. This reduction is due to increased proteasomal degradation following ubiquitination rather than reduction of mRNA expression or stability | Homo sapiens |