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Literature summary for 3.5.1.98 extracted from
- Properties of chicken erythrocyte histone deacetylase associated with the nuclear matrix (1996), Biochem. J., 314, 631-637.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | in Tris/HCl buffer, optimum concentration of NaCl is 50 mM for isoforms HD1 and HD2 and 100 mM for the nuclear matrix form | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Butyrate | non-competitive | Gallus gallus |  |
| additional information | in phosphate buffer, the presence of NaCl is inhibitory | Gallus gallus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nuclear matrix | associated to | Gallus gallus | 16363 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
isoform HD1, gel filtration | Gallus gallus |
| 220000 | - |
isoform HD2, gel filtration | Gallus gallus |
| 400000 | - |
enzyme complex solubilized from nuclear matrix, gel filtration | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
isoforms HD1 and HD2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N6-lysine acetylated histone H2A-H2B dimer + H2O | isoform HD2, slightly preferred substrate | Gallus gallus | histone H3-H4 dimer + acetate | - |
? | |
| N6-lysine acetylated histone H3-H4 tetramer + H2O | isoform HD1, preferred substrate | Gallus gallus | histone H3-H4 tetramer + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | isoform HD2 dissociates into HD1 when treated with 1.6 M NaCl or applied to a Q-Sepharose column. The active form of enzyme is a high-molecular-mass complex associated with proteins that are components of the nuclear matrix | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | isoform HD2 | Gallus gallus |
| 7 | 7.5 | nuclear matrix form of enzyme | Gallus gallus |
| 7.5 | - |
isoform HD1 | Gallus gallus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
isoform HD2 | Gallus gallus | Butyrate | |
| 0.045 | - |
isoform HD1 | Gallus gallus | Butyrate | |
| 0.08 | - |
nuclear matrix form of enzyme | Gallus gallus | Butyrate | |
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Release 2023.1 (January 2023)
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