Cloned (Comment) | Organism |
---|---|
gene pfmA, sequence comparisons, cloning of PfmA and recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pseudoalteromonas flavipulchra |
Protein Variants | Comment | Organism |
---|---|---|
H278S | site-directed mutagenesis, the mutant shows 80% reduced activity compared to wild-type | Pseudoalteromonas flavipulchra |
L279S | site-directed mutagenesis, the mutant shows 74% reduced activity compared to wild-type | Pseudoalteromonas flavipulchra |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | about 10% inhibition at 1 mM | Pseudoalteromonas flavipulchra | |
Ni2+ | about 10% inhibition at 1 mM | Pseudoalteromonas flavipulchra | |
SDS | complete inhibition at 1 mM | Pseudoalteromonas flavipulchra | |
Urea | 20% inhibition at 1 mM | Pseudoalteromonas flavipulchra | |
Zn2+ | about 10% inhibition at 1 mM | Pseudoalteromonas flavipulchra |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | PfmA lacks an N-terminal signal peptide and no activity is found in the cell medium | Pseudoalteromonas flavipulchra | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | increases the enzyme activity by 8.5% at 1 mM | Pseudoalteromonas flavipulchra | |
additional information | Na+, K+, Ca2+, Fe3+, and Cu2+ show no or poor effects on AHL acylase activity at 1 mM | Pseudoalteromonas flavipulchra |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
about, sequence calculation | Pseudoalteromonas flavipulchra |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudoalteromonas flavipulchra | the marine bacterium Pseudoalteromonas flavipulchra strain JG1 can degrade N-acylhomoserine lactones (AHLs). PfmA can degrade ampicillin but not penicillin G. The concentration of AHLs in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1 is reduced when 0.01 mg/ml PfmA is present | ? | - |
- |
|
additional information | Pseudoalteromonas flavipulchra JG1 | the marine bacterium Pseudoalteromonas flavipulchra strain JG1 can degrade N-acylhomoserine lactones (AHLs). PfmA can degrade ampicillin but not penicillin G. The concentration of AHLs in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1 is reduced when 0.01 mg/ml PfmA is present | ? | - |
- |
|
N-3-oxo-decanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra | - |
L-homoserine lactone + 3-oxodecanoate | - |
? | |
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra | - |
L-homoserine lactone + 3-oxododecanoate | - |
? | |
N-3-oxo-tetradecanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra | - |
L-homoserine lactone + 3-oxotetradecanoate | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra | - |
L-homoserine lactone + decanoate | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra JG1 | - |
L-homoserine lactone + decanoate | - |
? | |
N-dodecanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra | - |
L-homoserine lactone + dodecanoate | - |
? | |
N-dodecanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra JG1 | - |
L-homoserine lactone + dodecanoate | - |
? | |
N-tetradecanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra | - |
L-homoserine lactone + tetradecanoate | - |
? | |
N-tetradecanoyl-L-homoserine lactone + H2O | Pseudoalteromonas flavipulchra JG1 | - |
L-homoserine lactone + tetradecanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas flavipulchra | A0A223AMX9 | - |
- |
Pseudoalteromonas flavipulchra JG1 | A0A223AMX9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | post-translational modification is performed prior to PfmA becoming an active enzyme | Pseudoalteromonas flavipulchra |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudoalteromonas flavipulchra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the marine bacterium Pseudoalteromonas flavipulchra strain JG1 can degrade N-acylhomoserine lactones (AHLs). PfmA can degrade ampicillin but not penicillin G. The concentration of AHLs in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1 is reduced when 0.01 mg/ml PfmA is present | Pseudoalteromonas flavipulchra | ? | - |
- |
|
additional information | enzyme AHL acylase PfmA can degrade N-acylhomoserine lactones (AHLs) with acyl chains longer than 10 carbons. PfmA can degrade ampicillin but not penicillin G. No activity with N-hexanoyl-L-homoserine lactone, N-3-oxo-hexanoyl-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and N-3-oxo-octanoyl-L-homoserine lactone | Pseudoalteromonas flavipulchra | ? | - |
- |
|
additional information | the marine bacterium Pseudoalteromonas flavipulchra strain JG1 can degrade N-acylhomoserine lactones (AHLs). PfmA can degrade ampicillin but not penicillin G. The concentration of AHLs in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1 is reduced when 0.01 mg/ml PfmA is present | Pseudoalteromonas flavipulchra JG1 | ? | - |
- |
|
additional information | enzyme AHL acylase PfmA can degrade N-acylhomoserine lactones (AHLs) with acyl chains longer than 10 carbons. PfmA can degrade ampicillin but not penicillin G. No activity with N-hexanoyl-L-homoserine lactone, N-3-oxo-hexanoyl-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and N-3-oxo-octanoyl-L-homoserine lactone | Pseudoalteromonas flavipulchra JG1 | ? | - |
- |
|
N-3-oxo-decanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra | L-homoserine lactone + 3-oxodecanoate | - |
? | |
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra | L-homoserine lactone + 3-oxododecanoate | - |
? | |
N-3-oxo-tetradecanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra | L-homoserine lactone + 3-oxotetradecanoate | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra | L-homoserine lactone + decanoate | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra JG1 | L-homoserine lactone + decanoate | - |
? | |
N-dodecanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra | L-homoserine lactone + dodecanoate | - |
? | |
N-dodecanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra JG1 | L-homoserine lactone + dodecanoate | - |
? | |
N-tetradecanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra | L-homoserine lactone + tetradecanoate | - |
? | |
N-tetradecanoyl-L-homoserine lactone + H2O | - |
Pseudoalteromonas flavipulchra JG1 | L-homoserine lactone + tetradecanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 26000, alpha-subunit, + 1 * 68000, beta-subunit, His6-tagged recombinant enzyme, SDS-PAGE | Pseudoalteromonas flavipulchra |
More | second-structure analysis of PfmA shows that the protein includes an alpha subunit, a spacer sequence, and a beta subunit. The alpha subunit of PfmA is predicted to have seven alpha helices, two beta folds and three random coils, while the beta subunit is predicted to have 12 alpha helices, 19 beta folds and eight random coils | Pseudoalteromonas flavipulchra |
Synonyms | Comment | Organism |
---|---|---|
FaGL1422 | - |
Pseudoalteromonas flavipulchra |
N-acylhomoserine lactone acylase | - |
Pseudoalteromonas flavipulchra |
pfmA | - |
Pseudoalteromonas flavipulchra |
quorum-quenching N-acylhomoserine lactone acylase | - |
Pseudoalteromonas flavipulchra |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudoalteromonas flavipulchra |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | over 40% of maximal activity at 20-80°C, profile overview | Pseudoalteromonas flavipulchra |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | purified recombinant enzyme, completely stable at | Pseudoalteromonas flavipulchra |
40 | - |
purified recombinant enzyme, 40% activity remaining | Pseudoalteromonas flavipulchra |
50 | - |
purified recombinant enzyme, 30% activity remaining | Pseudoalteromonas flavipulchra |
60 | - |
purified recombinant enzyme, 10% activity remaining | Pseudoalteromonas flavipulchra |
70 | - |
purified recombinant enzyme, 5% activity remaining | Pseudoalteromonas flavipulchra |
80 | - |
purified recombinant enzyme, no activity remaining | Pseudoalteromonas flavipulchra |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudoalteromonas flavipulchra |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | over 50% of maximal activity at pH 5.0-11.0, 40% at pH 4.0, profile overview | Pseudoalteromonas flavipulchra |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme PfmA belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily and shows homology to a member of penicillin amidases, but PfmA can degrade ampicillin but not penicillin G | Pseudoalteromonas flavipulchra |
additional information | residue Ser256 in PfmA is the active site residue and residues Arg528, Asn324, His278 and Leu279 also play important roles in the catalytic process. Ser356 is also the first amino acid of the beta-subunit | Pseudoalteromonas flavipulchra |
physiological function | enzyme PfmA reduces N-acylhomoserine lactone (AHL) accumulation and the production of virulence factors in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1, and attenuates the virulence of Pseudomonas aeruginosa to increase Artemia survival, which suggests that PfmA can be considered as a therapeutic agent to control AHL-mediated pathogenicity. N-acylhomoserine lactone acylase is a quorum-quenching enzyme. Quorum sensing (QS) is a cell-to-cell communication mechanism dependent on cell density, which allows bacterial populations to regulate the expression of genes responsible for a wide range of biological behaviours, such as the production of pigments, biofilm formation, antibiotic production, motility, adhesion, bioluminescence and secretion of virulence factors | Pseudoalteromonas flavipulchra |