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Literature summary for 3.5.1.97 extracted from

  • Wahjudi, M.; Papaioannou, E.; Hendrawati, O.; van Assen, A.H.; van Merkerk, R.; Cool, R.H.; Poelarends, G.J.; Quax, W.J.
    PA0305 of Pseudomonas aeruginosa is a quorum quenching acylhomoserine lactone acylase belonging to the Ntn hydrolase superfamily (2011), Microbiology, 157, 2042-2055.
    View publication on PubMed
Search for more literature for 3.5.1.97

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Origami cells Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 1 * 26000 (C-terminally processed alpha-subunit) + 1 * 60000 (beta-subunit), SDS-PAGE Pseudomonas aeruginosa
60000
-
 1 * 26000 (C-terminally processed alpha-subunit) + 1 * 60000 (beta-subunit), SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity towards N-butanoyl-DL-homoserine lactone Pseudomonas aeruginosa ?
-
 ?
additional information PA0305 degrades N-acyl-homoserine lactones with acyl side chains ranging in length from 6 to 14 carbons Pseudomonas aeruginosa ?
-
 ?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
-
 Pseudomonas aeruginosa L-homoserine lactone + 3-oxodecanoate
-
 ?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
-
 Pseudomonas aeruginosa L-homoserine lactone + 3-oxododecanoate
-
 ?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
-
 Pseudomonas aeruginosa L-homoserine lactone + 3-oxohexanoate
-
 ?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
-
 Pseudomonas aeruginosa L-homoserine lactone + 3-oxooctanoate
-
 ?
N-(3-oxotetradecanoyl)-L-homoserine lactone + H2O
-
 Pseudomonas aeruginosa L-homoserine lactone + 3-oxotetradecanoate
-
 ?
N-acyl-(S)-homoserine lactone + H2O
-
 Pseudomonas aeruginosa (S)-homoserine lactone + carboxylic acid
-
 ?
N-decanoyl-DL-homoserine lactone + H2O
-
 Pseudomonas aeruginosa DL-homoserine lactone + decanoate
-
 ?
N-dodecanoyl-DL-homoserine lactone + H2O
-
 Pseudomonas aeruginosa DL-homoserine lactone + dodecanoate
-
 ?
N-heptanoyl-DL-homoserine lactone + H2O
-
 Pseudomonas aeruginosa DL-homoserine lactone + heptanoate
-
 ?
N-octanoyl-DL-homoserine lactone + H2O
-
 Pseudomonas aeruginosa DL-homoserine lactone + octanoate
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Pseudomonas aeruginosa L-homoserine lactone + octanoate
-
 ?
N-tetradecanoyl-DL-homoserine lactone + H2O
-
 Pseudomonas aeruginosa DL-homoserine lactone + tetradecanoate
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 26000 (C-terminally processed alpha-subunit) + 1 * 60000 (beta-subunit), SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
AHL acylase
-
 Pseudomonas aeruginosa
HacB
-
 Pseudomonas aeruginosa
PA0305
-
 Pseudomonas aeruginosa
quorum quenching acylhomoserine lactone acylase
-
 Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4
-
 N-octanoyl-L-homoserine lactone in 0.1 M phosphate buffer pH 7.4, at 30°C Pseudomonas aeruginosa
78
-
 N-(3-oxododecanoyl)-L-homoserine lactone in 0.1 M phosphate buffer pH 7.4, at 30°C Pseudomonas aeruginosa