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Literature summary for 3.5.1.97 extracted from

  • Chen, C.N.; Chen, C.J.; Liao, C.T.; Lee, C.Y.
    A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity (2009), BMC Microbiol., 9, 89.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli DH10B Ralstonia solanacearum GMI1000

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
SDS-PAGE, recombinant protein Ralstonia solanacearum GMI1000
88650
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predicted molecular mass of the recombinant protein Ralstonia solanacearum GMI1000

Organism

Organism UniProt Comment Textmining
Ralstonia solanacearum GMI1000
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an important soil-borne plant pathogen with an extensive host range
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Reaction

Reaction Comment Organism Reaction ID
an N-acyl-L-homoserine lactone + H2O = L-homoserine lactone + a carboxylate AHL-acylase is a quorum quenching enzyme and degrades N-acylhomoserine lactones (AHLs) by removing the fatty acid side chain from the homoserine lactone ring of AHLs. This blocks AHL accumulation and pathogenic phenotypes in quorum sensing bacteria Ralstonia solanacearum GMI1000

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a modified homoserine lactone-o-phthaldialdehyde (HSL-OPA) assay is used to quantify the recombinant AHL acylase activity. No activity is observed with short chain N-acylhomoserine lactones (AHLs), i.e. N-butanoyl-L-homoserine lactone, N-hexanoyl-L-homoserine, or N-(3-oxohexanoyl)-L-homoserine lactone. AHLs of more than ten carbon-acyl chains, i.e. N-decanoyl-L-homoserine lactone and N-dodecanoyl-L-homoserine lactone can not be determined due to their poor solubility Ralstonia solanacearum GMI1000 ?
-
?
additional information since the aac gene is predicted as a probable aculeacin A acylase aculeacin A is tested as substrate. The possible products i.e. palmatic acid is not found in the ESI-MS analysis Ralstonia solanacearum GMI1000 ?
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?
N-decanoyl-L-homoserine lactone + H2O
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Ralstonia solanacearum GMI1000 L-homoserine lactone + decanoic acid
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?
N-heptanoyl-L-homoserine lactone + H2O
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Ralstonia solanacearum GMI1000 L-homoserine lactone + heptanoic acid determined by ESI-MS analysis ?
N-octanoyl-L-homoserine lactone + H2O
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Ralstonia solanacearum GMI1000 L-homoserine lactone + octanoic acid
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?

Synonyms

Synonyms Comment Organism
AHL-acylase
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Ralstonia solanacearum GMI1000
N-acyl-homoserine lactone acylase encoded by the aac gene, a probable aculeacin A aclyase is a N-acyl-homoserine lactone acylase with quorum-quenching activity Ralstonia solanacearum GMI1000

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Ralstonia solanacearum GMI1000

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Ralstonia solanacearum GMI1000