Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli DH10B | Ralstonia solanacearum GMI1000 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
SDS-PAGE, recombinant protein | Ralstonia solanacearum GMI1000 |
88650 | - |
predicted molecular mass of the recombinant protein | Ralstonia solanacearum GMI1000 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia solanacearum GMI1000 | - |
an important soil-borne plant pathogen with an extensive host range | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an N-acyl-L-homoserine lactone + H2O = L-homoserine lactone + a carboxylate | AHL-acylase is a quorum quenching enzyme and degrades N-acylhomoserine lactones (AHLs) by removing the fatty acid side chain from the homoserine lactone ring of AHLs. This blocks AHL accumulation and pathogenic phenotypes in quorum sensing bacteria | Ralstonia solanacearum GMI1000 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a modified homoserine lactone-o-phthaldialdehyde (HSL-OPA) assay is used to quantify the recombinant AHL acylase activity. No activity is observed with short chain N-acylhomoserine lactones (AHLs), i.e. N-butanoyl-L-homoserine lactone, N-hexanoyl-L-homoserine, or N-(3-oxohexanoyl)-L-homoserine lactone. AHLs of more than ten carbon-acyl chains, i.e. N-decanoyl-L-homoserine lactone and N-dodecanoyl-L-homoserine lactone can not be determined due to their poor solubility | Ralstonia solanacearum GMI1000 | ? | - |
? | |
additional information | since the aac gene is predicted as a probable aculeacin A acylase aculeacin A is tested as substrate. The possible products i.e. palmatic acid is not found in the ESI-MS analysis | Ralstonia solanacearum GMI1000 | ? | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Ralstonia solanacearum GMI1000 | L-homoserine lactone + decanoic acid | - |
? | |
N-heptanoyl-L-homoserine lactone + H2O | - |
Ralstonia solanacearum GMI1000 | L-homoserine lactone + heptanoic acid | determined by ESI-MS analysis | ? | |
N-octanoyl-L-homoserine lactone + H2O | - |
Ralstonia solanacearum GMI1000 | L-homoserine lactone + octanoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHL-acylase | - |
Ralstonia solanacearum GMI1000 |
N-acyl-homoserine lactone acylase | encoded by the aac gene, a probable aculeacin A aclyase is a N-acyl-homoserine lactone acylase with quorum-quenching activity | Ralstonia solanacearum GMI1000 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ralstonia solanacearum GMI1000 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Ralstonia solanacearum GMI1000 |