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Literature summary for 3.5.1.97 extracted from

  • Shepherd, R.W.; Lindow, S.E.
    Two dissimilar N-acyl-homoserine lactone acylases of Pseudomonas syringae influence colony and biofilm morphology (2009), Appl. Environ. Microbiol., 75, 45-53.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.97

Application

Application Comment Organism
additional information fundamental roles in biofilm formation Pseudomonas syringae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas syringae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 alphabeta, 1 * 19000 + 1 * 60000, SDS-PAGE Pseudomonas syringae
22000
-
 alphabeta, 1 * 22000 + 1 * 600000, SDS-PAGE Pseudomonas syringae
60000
-
 alphabeta, 1 * 19000 + 1 * 60000, SDS-PAGE Pseudomonas syringae
600000
-
 alphabeta, 1 * 22000 + 1 * 600000, SDS-PAGE Pseudomonas syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae Q4ZV08 strain B728a, strain BHSL, strain HacA-, strain HacB-, strain HacA-B-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas syringae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0019
-
 substrate N-butyryl homoserine lactone Pseudomonas syringae
0.0049
-
 substrate N-(3-oxohexanoyl)-DL-homoserine lactone Pseudomonas syringae
0.0276
-
 substrate N-hexanoyl-DL-homoserine lactone Pseudomonas syringae
0.0406
-
 substrate N-decanoyl-DL-homoserine lactone Pseudomonas syringae
0.0408
-
 substrate N-(3-oxooctanoyl)-DL-homoserine lactone Pseudomonas syringae
0.0463
-
 substrate N-octanoyl-DL-homoserine lactone Pseudomonas syringae
1100
-
 substrate N-3-oxooctanoyl-DL-homoserine lactone Pseudomonas syringae
3200
-
 substrate N-octanoyl-DL-homoserine lactone Pseudomonas syringae
3800
-
 substrate N-decanoyl-DL-homoserine lactone Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(3-oxohexanoyl)-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + 3-oxohexanoate
-
 ?
N-(3-oxooctanoyl)-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + 3-oxooctanoate
-
 ?
N-3-oxooctanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 3 h Pseudomonas syringae homoserine lactone + 3-oxooctanoate
-
 ?
N-butyryl homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + butanoate
-
 ?
N-decanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + decanoate
-
 ?
N-decanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 3 h Pseudomonas syringae homoserine lactone + decanoate
-
 ?
N-dodecanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + dodecanoate
-
 ?
N-dodecanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 3 h Pseudomonas syringae homoserine lactone + dodecanoate
-
 ?
N-hexanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + hexanoate
-
 ?
N-octanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + octanoate
-
 ?
N-octanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 3 h Pseudomonas syringae homoserine lactone + octanoate
-
 ?

Subunits

Subunits Comment Organism
dimer alphabeta, 1 * 19000 + 1 * 60000, SDS-PAGE Pseudomonas syringae
dimer alphabeta, 1 * 22000 + 1 * 600000, SDS-PAGE Pseudomonas syringae

Synonyms

Synonyms Comment Organism
HacA
-
 Pseudomonas syringae
HacB
-
 Pseudomonas syringae
N-acyl-homoserine lactone acylase
-
 Pseudomonas syringae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Pseudomonas syringae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas syringae