Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 54% inhibition | Arthrobacter pascens | |
AgNO3 | 0.25 mM, complete inhibition | Arthrobacter pascens | |
CuCl | 0.25 mM, complete inhibition | Arthrobacter pascens | |
CuCl2 | 0.25 mM, complete inhibition | Arthrobacter pascens | |
dithiothreitol | 90% inhibition | Arthrobacter pascens | |
HgCl2 | 0.25 mM, complete inhibition | Arthrobacter pascens | |
N-ethylmaleimide | complete inhibition | Arthrobacter pascens | |
o-phenanthroline | 38% inhibition | Arthrobacter pascens | |
p-chloromercuribenzoate | complete inhibition | Arthrobacter pascens | |
phenylhydrazine | 25% inhibition | Arthrobacter pascens | |
SnCl2 | 0.25 mM, 34% inhibition | Arthrobacter pascens | |
ZnCl2 | 0.25 mM, 25% inhibition | Arthrobacter pascens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
N-benzylformamide | - |
Arthrobacter pascens | |
7.5 | - |
N-butylformamide | - |
Arthrobacter pascens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 2.94 mol per mol of subunit | Arthrobacter pascens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
2 * 61000, SDS-PAGE | Arthrobacter pascens |
121000 | - |
gel filtration | Arthrobacter pascens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzylformamide + H2O | Arthrobacter pascens | - |
formate + benzylamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter pascens | Q68AP4 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sephacel, Resource ISO, Ressource Q | Arthrobacter pascens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.9 | - |
- |
Arthrobacter pascens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzylformamide + H2O | - |
Arthrobacter pascens | formate + benzylamine | - |
? | |
N-butylformamide + H2O | 3.4% of activity with N-benzylformamide | Arthrobacter pascens | formate + butylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 61000, SDS-PAGE | Arthrobacter pascens |
Synonyms | Comment | Organism |
---|---|---|
NfdA | - |
Arthrobacter pascens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Arthrobacter pascens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
2% loss of activity after 30 min | Arthrobacter pascens |
35 | - |
6% loss of activity after 30 min | Arthrobacter pascens |
40 | - |
16% loss of activity after 30 min | Arthrobacter pascens |
45 | - |
25% loss of activity after 30 min | Arthrobacter pascens |
50 | - |
49% loss of activity after 30 min | Arthrobacter pascens |
55 | - |
complete loss of activity after 30 min | Arthrobacter pascens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arthrobacter pascens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | stable | Arthrobacter pascens |