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Literature summary for 3.5.1.91 extracted from

  • Fukatsu, H.; Hashimoto, Y.; Goda, M.; Higashibata, H.; Kobayashi, M.
    Amine-synthesizing enzyme N-substituted formamide deformylase: screening, purification, characterization, and gene cloning (2004), Proc. Natl. Acad. Sci. USA, 101, 13726-13731.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 54% inhibition Arthrobacter pascens
AgNO3 0.25 mM, complete inhibition Arthrobacter pascens
CuCl 0.25 mM, complete inhibition Arthrobacter pascens
CuCl2 0.25 mM, complete inhibition Arthrobacter pascens
dithiothreitol 90% inhibition Arthrobacter pascens
HgCl2 0.25 mM, complete inhibition Arthrobacter pascens
N-ethylmaleimide complete inhibition Arthrobacter pascens
o-phenanthroline 38% inhibition Arthrobacter pascens
p-chloromercuribenzoate complete inhibition Arthrobacter pascens
phenylhydrazine 25% inhibition Arthrobacter pascens
SnCl2 0.25 mM, 34% inhibition Arthrobacter pascens
ZnCl2 0.25 mM, 25% inhibition Arthrobacter pascens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
N-benzylformamide
-
Arthrobacter pascens
7.5
-
N-butylformamide
-
Arthrobacter pascens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 2.94 mol per mol of subunit Arthrobacter pascens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
2 * 61000, SDS-PAGE Arthrobacter pascens
121000
-
gel filtration Arthrobacter pascens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-benzylformamide + H2O Arthrobacter pascens
-
formate + benzylamine
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter pascens Q68AP4
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephacel, Resource ISO, Ressource Q Arthrobacter pascens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.9
-
-
Arthrobacter pascens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzylformamide + H2O
-
Arthrobacter pascens formate + benzylamine
-
?
N-butylformamide + H2O 3.4% of activity with N-benzylformamide Arthrobacter pascens formate + butylamine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 61000, SDS-PAGE Arthrobacter pascens

Synonyms

Synonyms Comment Organism
NfdA
-
Arthrobacter pascens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Arthrobacter pascens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
2% loss of activity after 30 min Arthrobacter pascens
35
-
6% loss of activity after 30 min Arthrobacter pascens
40
-
16% loss of activity after 30 min Arthrobacter pascens
45
-
25% loss of activity after 30 min Arthrobacter pascens
50
-
49% loss of activity after 30 min Arthrobacter pascens
55
-
complete loss of activity after 30 min Arthrobacter pascens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Arthrobacter pascens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8.5 stable Arthrobacter pascens