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Literature summary for 3.5.1.90 extracted from

  • Gray, M.J.; Escalante-Semerena, J.C.
    The cobinamide amidohydrolase (cobyric acid-forming) CbiZ enzyme: a critical activity of the cobamide remodelling system of Rhodobacter sphaeroides (2009), Mol. Microbiol., 74, 1198-1210.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.90

Cloned(Commentary)

Cloned (Comment) Organism
the CbiZ protein is isolated as a fusion protein with N-terminal maltose-binding protein MBP an His6 tags and overproduced in Escherichia coli BL21 strain Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the CbiZ protein is isolated as a fusion protein with N-terminal maltose-binding protein MBP an His6 tags and overproduced in Escherichia coli BL21 strain. The recombinant protein is purified Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 pH 10, 50°C, adenosylcobalamide as substrate Cereibacter sphaeroides
0.07
-
 pH 10, 50°C, adenosylpseudocobalamide as substrate Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosylpseudocobalamin + H2O Rhodobacter sphaeroides requires cobalamin to grow on acetate. It has a CbiZ-dependent mechanism for the conversion of pseudocobalamin into cobalamin Cereibacter sphaeroides ?
-
 ?
adenosylpseudocobalamine + H2O the enzyme uses adenosylpseudocobalamin as a substrate but not adenosylcobalamin Cereibacter sphaeroides adenosylcobyric acid + (R)-1-aminopropan-2-ol formation of product monitored by HPLC, product not formed in the absence of dithiothreitol ?

Synonyms

Synonyms Comment Organism
CbiZ
-
 Cereibacter sphaeroides
cobinamide amidohydrolase (cobyric acid-forming)
-
 Cereibacter sphaeroides
MBP-CbiZ protein the CbiZ protein is isolated as a fusion protein with N-terminal maltose binding protein (MBP) and His6 tags Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 at pH 10, product formation is linear over time up to 15 min with 1 or 2 microg MBP-CbiZ protein in the reaction mixture Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 at 30°C Cereibacter sphaeroides

General Information

General Information Comment Organism
physiological function the activity is essential for the conversion of adenosylpseudocobalamin into adenosylcobalamin, the cobinamide needed for the catabolism of acetate. The physiological role of CbiZ in Rhodobacter sphaeroides is to remove the lower ligand of adenosylpseudocobalamin so that the cell can replace the lower ligand with 5,6-dimethylbenzimidazole, generating adenosylcobalamin Cereibacter sphaeroides