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Literature summary for 3.5.1.90 extracted from
- The cbiS gene of the archaeon Methanopyrus kandleri AV19 encodes a bifunctional enzyme with adenosylcobinamide amidohydrolase and alpha-ribazole-phosphate phosphatase activities (2006), J. Bacteriol., 188, 4227-4235.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| MgCl2 | activity is enhanced 2.7fold by adding 10 mM MgCl2 to the reaction mixture | Methanopyrus kandleri |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli and Salmonella enterica | Methanopyrus kandleri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
monomer, SDS-PAGE | Methanopyrus kandleri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanopyrus kandleri | - |
AV19 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanopyrus kandleri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.009 | - |
(CN)2-cobinamide-GDP as substrate | Methanopyrus kandleri |
| 0.009 | - |
cyanocobalamin as substrate | Methanopyrus kandleri |
| 0.02 | - |
adenosylcobinamide and (CN)2-cobinamide as substrate | Methanopyrus kandleri |
| 0.027 | - |
(CN)2-cobinamide-P as substrate | Methanopyrus kandleri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (CN)2-cobinamide + H2O | - |
Methanopyrus kandleri | adenosylcobyric acid + ? | - |
? | |
| (CN)2-cobinamide-GDP + H2O | - |
Methanopyrus kandleri | adenosylcobyric acid + ? | - |
? | |
| (CN)2-cobinamide-P + H2O | - |
Methanopyrus kandleri | adenosylcobyric acid + ? | - |
? | |
| adenosylcobinamide + H2O | pH 9.0, anaerobical incubation at 90°C for 1 h | Methanopyrus kandleri | adenosylcobyric acid + (R)-1-aminopropan-2-ol | - |
? | |
| cyanocobalamin + H2O | - |
Methanopyrus kandleri | adenosylcobyric acid + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenosylcobinamide amidohydrolase | - |
Methanopyrus kandleri |
| AdoCbi amidohydrolase | - |
Methanopyrus kandleri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | 100 | - |
Methanopyrus kandleri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Methanopyrus kandleri |
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Release 2023.1 (January 2023)
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