Cloned (Comment) | Organism |
---|---|
gene kynB or SCO3644, gene expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
N-formyl-L-kynurenine | pH 7.4, temperature not specified in the publication, recombinant His6-tagged enzyme | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-kynurenine + H2O | Streptomyces coelicolor | - |
formate + L-kynurenine | - |
? | |
N-formyl-L-kynurenine + H2O | Streptomyces coelicolor M145 | - |
formate + L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9X8R1 | gene SCO3644; gene kynB or SCO3644 | - |
Streptomyces coelicolor M145 | Q9X8R1 | gene SCO3644; gene kynB or SCO3644 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration | Streptomyces coelicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
pH 7.4, temperature not specified in the publication, purified recombinant His6-tagged enzyme | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-kynurenine + H2O | - |
Streptomyces coelicolor | formate + L-kynurenine | - |
? | |
N-formyl-L-kynurenine + H2O | - |
Streptomyces coelicolor M145 | formate + L-kynurenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KFA | - |
Streptomyces coelicolor |
kynurenine formamidase | - |
Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Streptomyces coelicolor |
Organism | Comment | Expression |
---|---|---|
Streptomyces coelicolor | tryptophan positively affects kynB transcription | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the tryptophan-to-anthranilate pathway | Streptomyces coelicolor |