Literature summary for 3.5.1.9 extracted from

Dobrovolsky, V.N.; Bowyer, J.F.; Pabarcus, M.K.; Heflich, R.H.; Williams, L.D.; Doerge, D.R.; Arvidsson, B.; Bergquist, J.; Casida, J.E.
Effect of arylformamidase (kynurenine formamidase) gene inactivation in mice on enzymatic activity, kynurenine pathway metabolites and phenotype (2005), Biochim. Biophys. Acta, 1724, 163-172.

Search for more literature for 3.5.1.9

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of heterozygous and knockout enzyme-deficient mutants, inactivation through the removal of a shared bidirectional promoter region regulating expression of the Afmid gene and the thymidine kinase gene, phenotype overview, Afmid knockout mice show 13% residual enzyme activity in kidney due to a distinct enzyme activity	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mus musculus	an enzyme other than the aryl formamidase additionally catalyzes the formation of L-kynurenine in kidney	?	-	?
N-formyl-L-kynurenine + H2O	Mus musculus	step in tryptophan metabolism and biosynthesis of kynurenine-derived derivatives, e.g. kynurenic acid, quinolinic acid, nicotinamide, NAD+, and NADP+, pathway disruption causes neurotoxicity and immunotoxicity, overview	formate + L-kynurenine	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	gene Afmid	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Mus musculus	-
liver	high activity	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	determination of kynurenine pathway metabolites concentrations in plasma of wild-type and mutant mice	Mus musculus
0.00002	-	liver knockout mutant enzyme	Mus musculus
0.00087	-	kidney knockout mutant enzyme	Mus musculus
0.0038	-	kidney heterozygous mutant enzyme	Mus musculus
0.0067	-	kidney wild-type enzyme	Mus musculus
0.0083	-	liver heterozygous mutant enzyme	Mus musculus
0.0156	-	liver wild-type enzyme	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	an enzyme other than the aryl formamidase additionally catalyzes the formation of L-kynurenine in kidney	Mus musculus	?	-	?
N-formyl-L-kynurenine + H2O	-	Mus musculus	formate + L-kynurenine	-	?
N-formyl-L-kynurenine + H2O	step in tryptophan metabolism and biosynthesis of kynurenine-derived derivatives, e.g. kynurenic acid, quinolinic acid, nicotinamide, NAD+, and NADP+, pathway disruption causes neurotoxicity and immunotoxicity, overview	Mus musculus	formate + L-kynurenine	-	?

Synonyms

Synonyms	Comment	Organism
AFMID	-	Mus musculus
kynurenine formamidase	-	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Mus musculus

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Release 2023.1 (January 2023)