Cloned (Comment) | Organism |
---|---|
gene def, recombinant expression of the enzyme, containing an N-terminal His6-tag followed by a Tobacco etch virus protease cleavage site and three alanine residues before the NdeI site to facilitate purification of the expressed protein, in Escherichia coli strain BL21(DE3) | Campylobacter jejuni |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged enzyme, hanging drop vapour diffusion method, mixing of 900 nl of 6 mg/ml protein in 20 mM TrisHCl pH 8.0, 20 mM NaCl, 3 mM 2-mercaptoethanol, with 900 nl reservoir solution containing 2 M zinc acetate dihydrate, 20% w/v PEG 3350, pH 6.4, and euilibration against 1 ml reservoir solution, microseeding and buffer optimization, 4 days, X-ray diffraction structure determination and analysis at 2.9 A resolution | Campylobacter jejuni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene def | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, cation exchange chromatography and dialysis | Campylobacter jejuni |