Literature summary for 3.5.1.88 extracted from

Che, X.; Hu, J.; Wang, L.; Zhu, Z.; Xu, Q.; Lv, J.; Fu, Z.; Sun, Y.; Sun, J.; Lin, G.; Lu, R.; Yao, Z.
Expression, purification, and activity assay of peptide deformylase from Escherichia coli and Staphylococcus aureus (2011), Mol. Cell. Biochem., 357, 47-54.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Staphylococcus aureus

General Stability

General Stability	Organism
PDF has high activity in Tris buffer, but low activity in HEPES and NaH2PO4 buffers	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
actinonin	actinonin does exert a significant inhibitory effect on enzyme activity in the presence of 0.25 mM substrate (complete inhibition at 10 nM)	Staphylococcus aureus
additional information	there is no significant inhibitory effect of actinonin on enzyme activity when the concentration of substrate is 4 mM	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the imidazole ring of histidine can combine with a metal ion such as iron. The activity of the iron form of the enzyme is higher than the nickel form	Staphylococcus aureus
Ni2+	the imidazole ring of histidine can combine with a metal ion such as nickel. The activity of the iron form of the enzyme is higher than the nickel form	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	x * 20000, SDS-PAGE	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-
Staphylococcus aureus Mu50	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography and Sephacryl S-300 gel filtration	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-formyl-Met-Val-Ser + H2O	-	Staphylococcus aureus	formate + Met-Val-Ser	-	?
N-formyl-Met-Val-Ser + H2O	-	Staphylococcus aureus Mu50	formate + Met-Val-Ser	-	?

Subunits

Subunits	Comment	Organism
?	x * 20000, SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
PDF	-	Staphylococcus aureus

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Release 2023.1 (January 2023)