Application | Comment | Organism |
---|---|---|
medicine | possibility of future design of novel mycobacteria-specific peptide deformylase inhibitors | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
into a pET-vector for expression in Escherichia coli Bl21DE3 cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
R77A | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R77D | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R77K | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R77K/R78K/R79K | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R78A | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R78D | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R78K | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R79A | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R79D | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
R79k | mutant, constructed to demonstrate that the three arginines are important for the activity of mPDF | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-phosphothiorate modified antisense oligodeoxyribonucleotides | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
formyl-Met-Ala | mutant R79A | Mycobacterium tuberculosis | |
4.1 | - |
formyl-Met-Ala | wild-type | Mycobacterium tuberculosis | |
5 | - |
formyl-Met-Ala | mutant R79K | Mycobacterium tuberculosis | |
5.6 | - |
formyl-Met-Ala | mutant R78K | Mycobacterium tuberculosis | |
6.4 | - |
formyl-Met-Ala | mutant R78A | Mycobacterium tuberculosis | |
6.5 | - |
formyl-Met-Ala | mutant R77A | Mycobacterium tuberculosis | |
6.9 | - |
formyl-Met-Ala | mutant R77K | Mycobacterium tuberculosis | |
9.3 | - |
formyl-Met-Ala | mutant R77D | Mycobacterium tuberculosis | |
11.7 | - |
formyl-Met-Ala | mutant R78D | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Mycobacterium tuberculosis | |
Zn2+ | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22600 | - |
- |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | peptide deformylase is involved in the deformylation of the N-formyl group of newly synthesized polypeptides during protein synthesis | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Ra / ATCC 25177 | peptide deformylase is involved in the deformylation of the N-formyl group of newly synthesized polypeptides during protein synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on a Ni2+-nitrilotriacetic acid column | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-Met-Ala + H2O | substrate activity assay | Mycobacterium tuberculosis | formate + Met-Ala | - |
? | |
formyl-Met-Ala + H2O | substrate activity assay | Mycobacterium tuberculosis H37Ra / ATCC 25177 | formate + Met-Ala | - |
? | |
additional information | peptide deformylase is involved in the deformylation of the N-formyl group of newly synthesized polypeptides during protein synthesis | Mycobacterium tuberculosis | ? | - |
? | |
additional information | peptide deformylase is involved in the deformylation of the N-formyl group of newly synthesized polypeptides during protein synthesis | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mPDF | - |
Mycobacterium tuberculosis |
peptide deformylase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
formyl-Met-Ala | mutant R79A | Mycobacterium tuberculosis | |
0.24 | - |
formyl-Met-Ala | mutant R79K | Mycobacterium tuberculosis | |
0.6 | - |
formyl-Met-Ala | mutant R77D | Mycobacterium tuberculosis | |
0.8 | - |
formyl-Met-Ala | mutant R78D | Mycobacterium tuberculosis | |
1.6 | - |
formyl-Met-Ala | mutant R77A | Mycobacterium tuberculosis | |
1.6 | - |
formyl-Met-Ala | mutant R78K | Mycobacterium tuberculosis | |
1.7 | - |
formyl-Met-Ala | mutant R78A | Mycobacterium tuberculosis | |
1.8 | - |
formyl-Met-Ala | mutant R77K | Mycobacterium tuberculosis | |
5 | - |
formyl-Met-Ala | wild-type | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Mycobacterium tuberculosis |