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Literature summary for 3.5.1.88 extracted from

  • Robien, M.A.; Nguyen, K.T.; Kumar, A.; Hirsh, I.; Turley, S.; Pei, D.; Hol, W.G.
    An improved crystal form of Plasmodium falciparum peptide deformylase (2004), Protein Sci., 13, 1155-1163.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.88

Application

Application Comment Organism
medicine potential drug target, antimalarial drugs Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
into pET29b and pET22b vector for expression in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
2.2 A, cocrystallized with a synthesized inhibitor - 2.8 A resolution of the unliganded PfPDF Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
Ndelta57PfPDF Cdelta234 PfPDF variant with truncations of the first aminoterminal 57 acid residues and carboxyl terminus to amino acid residue S234 Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formyl-L-methionyl peptide + H2O Plasmodium falciparum
-
 formate + methionyl peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified by cation exchange chromatography followed by Co-NTA affinity column chromatography Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyl-L-methionyl peptide + H2O
-
 Plasmodium falciparum formate + methionyl peptide
-
 ?

Synonyms

Synonyms Comment Organism
peptide deformylase
-
 Plasmodium falciparum
PfPDF
-
 Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 for purified and modified PfPDF Plasmodium falciparum