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Literature summary for 3.5.1.88 extracted from

  • Sonke, T.; Kaptein, B.; Wagner, A.F.; Quaedflieg, P.J.; Schultz, S.; Ernste, S.; Schepers, A.; Mommers, J.H.; Broxterman, Q.B.
    Peptide deformylase as biocatalyst for the synthesis of enantiomerically pure amino acid derivatives (2004), J. Mol. Catal. B, 29, 265-277.
No PubMed abstract available
Search for more literature for 3.5.1.88

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formyl-L-methionyl peptide + H2O Escherichia coli
-
 formate + methionyl peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
based of one affinity chromatography step, 200 mg of pure Escherichia coli peptide deformylase, EcPDF, were yielded from 1l of culture Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(RS)-phenylalanine nitrile + formate
-
 Escherichia coli (S)-N-formyl-phenylalanine nitrile 99% (S)-enantiomer r
formyl-L-methionyl peptide + H2O
-
 Escherichia coli formate + methionyl peptide
-
 ?
N-formyl-1-(1-naphthyl)ethylamine + H2O
-
 Escherichia coli (1R)-1-naphthalen-1-ylethanamine + formate 90% (R)-enantiomer ?
N-formyl-3-amino-3-phenylpropionic acid + H2O
-
 Escherichia coli (R)-3-amino-3-phenylpropionic acid + formate 100% (R)-enantiomer ?
N-formyl-alaninol + H2O
-
 Escherichia coli (S)-alaninol + formate 85.6% (S)-enantiomer ?
N-formyl-alpha-methyl-phenylglycine amide + H2O
-
 Escherichia coli (S)-alpha-methyl-phenylglycine amide + formate 100% (S)-enantiomer ?
N-formyl-Leu-Tle-NHCH3 + H2O Tle i.e. tert-leucine Escherichia coli (S)-Leu-(S)-Tle-NHCH3 Tle i.e. tert-leucine, 94% (S,S)-diastereomer ?
N-formyl-m-methoxyphenylalanine nitrile + H2O
-
 Escherichia coli (S)-m-methoxyphenylalanine nitrile + formate 99% (S)-enantiomer ?
N-formyl-Met-Ala + H2O
-
 Escherichia coli Met-Ala + formate
-
 ?
N-formyl-phenylalanine nitrile + H2O
-
 Escherichia coli (S)-phenylalanine nitrile + formate 98.8% (S)-enantiomer ?
N-formyl-phenylglycine + H2O
-
 Escherichia coli (S)-phenylglycine + formate 99.6% (S)-enantiomer ?
N-formyl-phenylglycine amide + H2O
-
 Escherichia coli (S)-phenylglycine amide + formate 99.7% (S)-enantiomer ?
N-formyl-phenylglycinol + H2O
-
 Escherichia coli (S)-phenylglycinol + formate 90.5-93.3% (S)-enantiomer, depending on method ?
N-formyl-tert-leucine amide + H2O
-
 Escherichia coli (S)-tert-leucine amide + formate 100% (S)-enantiomer ?
N-formyl-valine nitrile + H2O
-
 Escherichia coli (S)-valine nitrile + formate 98.8% (S)-enantiomer ?

Synonyms

Synonyms Comment Organism
EcPDF
-
 Escherichia coli
PDF
-
 Escherichia coli
peptide deformylase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.045
-
 N-formyl-alpha-methyl-phenylglycine amide pH 7.2, 37°C Escherichia coli
0.15
-
 N-formyl-tert-leucine amide pH 7.2, 37°C Escherichia coli
0.22
-
 N-formyl-alaninol pH 7.2, 37°C Escherichia coli
0.45
-
 N-formyl-1-(1-naphthyl)ethylamine pH 7.2, 37°C Escherichia coli
0.69 6.3 N-formyl-phenylglycinol pH 7.2, 37°C Escherichia coli
6.08
-
 N-formyl-phenylglycinol pH 7.2, 37°C Escherichia coli
7.1
-
 N-formyl-3-amino-3-phenylpropionic acid pH 7.2, 37°C Escherichia coli
10.6
-
 N-formyl-phenylglycine pH 7.2, 37°C Escherichia coli
29.7
-
 N-formyl-valine nitrile pH 7.2, 37°C Escherichia coli
227
-
 N-formyl-phenylglycine amide pH 7.2, 37°C Escherichia coli
880
-
 N-formyl-phenylalanine nitrile pH 7.2, 37°C Escherichia coli
1370
-
 N-formyl-m-methoxyphenylalanine nitrile pH 7.2, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 formylation Escherichia coli
7.2
-
 deformylation Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.1 11.2 deformylation, nearly constant activity in this range Escherichia coli