Literature summary for 3.5.1.88 extracted from

Han, C.; Wang, Q.; Dong, L.; Sun, H.; Peng, S.; Chen, J.; Yang, Y.; Yue, J.; Shen, X.; Jiang, H.
Molecular cloning and characterization of a new peptide deformylase from human pathogenic bacterium Helicobacter pylori (2004), Biochem. Biophys. Res. Commun., 319, 1292-1298.

Search for more literature for 3.5.1.88

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Helicobacter pylori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7	-	formyl-Met-Ala-Ser	pH 7.5, 37°C, Co2+-containing enzyme	Helicobacter pylori

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	the activity of Co2+-containing enzyme is higher than the activity of Zn2+-containing enzyme	Helicobacter pylori
Zn2+	the activity of Co2+-containing enzyme is higher than the activity of Zn2+-containing enzyme	Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20970	-	x * 20970, mass spectrometry	Helicobacter pylori
20973	-	x * 20973, calculation from nucleotide sequence	Helicobacter pylori

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	Q672W7	-	-
Helicobacter pylori SS1	Q672W7	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Helicobacter pylori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formyl-Met-Ala-Ser + H2O	-	Helicobacter pylori	formate + Met-Ala-Ser	-	?
formyl-Met-Ala-Ser + H2O	-	Helicobacter pylori SS1	formate + Met-Ala-Ser	-	?

Subunits

Subunits	Comment	Organism
?	x * 20970, mass spectrometry	Helicobacter pylori
?	x * 20973, calculation from nucleotide sequence	Helicobacter pylori

Synonyms

Synonyms	Comment	Organism
HpPDF	-	Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Helicobacter pylori

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	60	40°C: about 50% of maximal activity, 60°C, about 70% of maximal activity	Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.4	-	formyl-Met-Ala-Ser	pH 7.5, 37°C, Co2+-containing enzyme	Helicobacter pylori

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	9	-	Helicobacter pylori

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	11	pH 6.5: about 55% of maximal activity, pH 11.0: about 90% of maximal activity	Helicobacter pylori

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Release 2023.1 (January 2023)