Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.88 extracted from

  • Lee, M.D.; Antczak, C.; Li, Y.; Sirotnak, F.M.; Bornmann, W.G.; Scheinberg, D.A.
    A new human peptide deformylase inhibitable by actinonin (2003), Biochem. Biophys. Res. Commun., 312, 309-315.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant lacking the first 63 amino acids is expressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
actinonin competitive, IC50: 43 nM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
formyl-Met-Leu-p-nitroanilide
-
Homo sapiens
11
-
formyl-Met-Ala-Ser
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the Co2+-containing enzyme form is active Homo sapiens
additional information Ni2+ and Zn2+-containing enzyme forms are inactive Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HL-60 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyl-Met-Ala-Ser + H2O
-
Homo sapiens formate + Met-Ala-Ser
-
?
formyl-Met-Leu-p-nitroanilide + H2O
-
Homo sapiens formate + Met-Leu-p-nitroanilide
-
?
additional information no detectable activity with formyl-Met-Leu-Phe and formyl-Met-Leu-Pro Homo sapiens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 50
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00046
-
formyl-Met-Leu-p-nitroanilide
-
Homo sapiens
0.0081
-
formyl-Met-Ala-Ser
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.2
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000043
-
competitive, IC50: 43 nM Homo sapiens actinonin