Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.88 extracted from

  • Haas, M.; Beyer, D.; Gahlmann, R.; Freiberg, C.
    YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases (2001), Microbiology, 147, 1783-1791.
    View publication on PubMed

Application

Application Comment Organism
medicine attractive target for antibacterial drug discovery Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
Def and YkrB, expression in Escherichia coli Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
actinonin 50% inhibition of enzyme Def at 95 nM, 50% inhibition of enzyme form YkrB by 130 nM Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
N-formyl-Met-Ala-Ser enzyme YkrB Bacillus subtilis
3
-
N-formyl-Met-Ala-Ser enzyme Def Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
harbours two functional peptide deformylase: Def and YkrB. YkrB probably is the predominant deformylase
-
Pseudomonas aeruginosa
-
two functional deformylases
-
Streptococcus pneumoniae
-
two functional deformylases
-
Streptococcus pyogenes
-
two functional deformylases
-

Purification (Commentary)

Purification (Comment) Organism
Def and YkrB, recombinant enzyme Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formyl-Met-Ala-Ser + H2O
-
Bacillus subtilis formate + Met-Ala-Ser
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
N-formyl-Met-Ala-Ser enzyme Def Bacillus subtilis
0.17
-
N-formyl-Met-Ala-Ser enzyme YkrB Bacillus subtilis