Application | Comment | Organism |
---|---|---|
medicine | attractive target for antibacterial drug discovery | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
Def and YkrB, expression in Escherichia coli | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinonin | 50% inhibition of enzyme Def at 95 nM, 50% inhibition of enzyme form YkrB by 130 nM | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
N-formyl-Met-Ala-Ser | enzyme YkrB | Bacillus subtilis | |
3 | - |
N-formyl-Met-Ala-Ser | enzyme Def | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
harbours two functional peptide deformylase: Def and YkrB. YkrB probably is the predominant deformylase | - |
Pseudomonas aeruginosa | - |
two functional deformylases | - |
Streptococcus pneumoniae | - |
two functional deformylases | - |
Streptococcus pyogenes | - |
two functional deformylases | - |
Purification (Comment) | Organism |
---|---|
Def and YkrB, recombinant enzyme | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-Met-Ala-Ser + H2O | - |
Bacillus subtilis | formate + Met-Ala-Ser | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
N-formyl-Met-Ala-Ser | enzyme Def | Bacillus subtilis | |
0.17 | - |
N-formyl-Met-Ala-Ser | enzyme YkrB | Bacillus subtilis |