Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
General Stability | Organism |
---|---|
enhanced stabilitiy in concentrated form | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | inactivation due to oxidation of Fe2+ to Fe3+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | natural metal, bound to active site | Escherichia coli | |
Fe2+ | sensitive to reactive oxygen | Escherichia coli | |
Zn2+ | Zn2+-bound form not sensitive to oxidation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19200 | - |
gel filtration, electrospray ionization mass spectrometry | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
presence of H2O2 and O2 leads to inactivation, enzymatic exclusion of O2 renders the deformylase highly stable. Zn2+-bound enzyme form is not sensitive to oxidation | Escherichia coli |
sensitive to intracellular reactive oxygen | Escherichia coli |
Purification (Comment) | Organism |
---|---|
to homogeneity, improved technique for protection to oxidation | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-Met-Leu-p-nitroanilide + H2O | - |
Escherichia coli | formate + Met-Leu-p-nitroanilide | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
t1/2 is 1 min | Escherichia coli |