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Literature summary for 3.5.1.87 extracted from

  • Duan, J.; Zhang, Q.; Zhao, H.; Du, J.; Bai, F.; Bai, G.
    Cloning, expression, characterization and application of atcA, atcB and atcC from Pseudomonas sp. for the production of L-cysteine (2012), Biotechnol. Lett., 34, 1101-1106.
    View publication on PubMed
Search for more literature for 3.5.1.87

Cloned(Commentary)

Cloned (Comment) Organism
gene atcC, organized in the L-cysteine synthesis gene cluster, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, recombinant enzyme, SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-carbamoyl-L-cysteine + H2O Pseudomonas sp.
-
 L-cysteine + CO2 + NH3
-
 ?
N-carbamoyl-L-cysteine + H2O Pseudomonas sp. QR-101
-
 L-cysteine + CO2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. H9B8T5 isolated from industrial waste water, gene atcC
-
Pseudomonas sp. QR-101 H9B8T5 isolated from industrial waste water, gene atcC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-L-cysteine + H2O
-
 Pseudomonas sp. L-cysteine + CO2 + NH3
-
 ?
N-carbamoyl-L-cysteine + H2O
-
 Pseudomonas sp. QR-101 L-cysteine + CO2 + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, recombinant enzyme, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
AtcC
-
 Pseudomonas sp.
L-NCC amidohydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 recombinant enzyme Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Pseudomonas sp.

General Information

General Information Comment Organism
metabolism the enzyme is involved in the (N-carbamoyl-L-cysteine) pathway (L-NCC pathway) converting DL-2-amino-DELTA2-thiazoline-4-carboxylic acid to L-cysteine Pseudomonas sp.