Application | Comment | Organism |
---|---|---|
additional information | immobilization of the enzyme by covalent coupling to a solid support material including additional cross-linking with polyaldehyde-dextran, method development, overview. Temperature and pH optima of immobilized enzyme are increased by 10°C and 0.5 unit, respectively. The enzyme is significantly stabilized, it is recycled nine times with about 100% conversion efficiency when batch experiments are carried out at 35°C, pH 7.5, for the 180 min cycle | Brevibacillus reuszeri |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the enzyme by covalent coupling to a solid support material including additional cross-linking with polyaldehyde-dextran, method development, overview. Temperature and pH optima of immobilized enzyme are increased by 10°C and 0.5 unit, respectively. The enzyme is significantly stabilized, it is recycled nine times with about 100% conversion efficiency when batch experiments are carried out at 35°C, pH 7.5, for the 180 min cycle | Brevibacillus reuszeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.8 | - |
N-carbamoyl-L-methionine | pH 7.5, 40°C | Brevibacillus reuszeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-L-methionine + H2O | Brevibacillus reuszeri | - |
L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | Brevibacillus reuszeri HSN1 | - |
L-methionine + CO2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus reuszeri | - |
- |
- |
Brevibacillus reuszeri HSN1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 60fold to homogeneity by heat treatment at 70°C for 2 min, anion exchange chromatography of the supernatant, hydrophobic interaction chromatography, ultrafiltration, and gel filtration | Brevibacillus reuszeri |
Storage Stability | Organism |
---|---|
4°C, the immobilized enzyme retaines approximately 50% of its activity after 5 months, while the free enzyme loses the complete enzyme activity with a half-life of 12 days | Brevibacillus reuszeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-L-methionine + H2O | - |
Brevibacillus reuszeri | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | preferred substrate | Brevibacillus reuszeri | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | - |
Brevibacillus reuszeri HSN1 | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | preferred substrate | Brevibacillus reuszeri HSN1 | L-methionine + CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | - |
Brevibacillus reuszeri |
Synonyms | Comment | Organism |
---|---|---|
L-methionine-N-carbamoylase | - |
Brevibacillus reuszeri |
L-N-carbamoylase | - |
Brevibacillus reuszeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free enzyme | Brevibacillus reuszeri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the immobilized enzyme retains about 93% activity after 60 min, at pH 7.5, whereas the free enzyme loses about 50% activity | Brevibacillus reuszeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
free enzyme | Brevibacillus reuszeri |