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Literature summary for 3.5.1.87 extracted from
- New gene cluster from the thermophile Bacillus fordii MH602 in the conversion of DL-5-substituted hydantoins to L-amino acids (2009), J. Microbiol. Biotechnol., 19, 1497-1505.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 | Siminovitchia fordii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
SDS-PAGE | Siminovitchia fordii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Siminovitchia fordii | - |
MH602, thermophile bacterium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the activity of the recombinant enzyme is lower than the wild-type strain MH602. The expressed proteins are mainly present in the insoluble fraction, indicating the formation of inclusion bodies | Siminovitchia fordii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-carbamoyl-L-phenylalanine + H2O | the recombinant L-carbamoylase from Escherichia coli harboring the pET-Case is L-stereospecific | Siminovitchia fordii | L-phenylalanine + CO2 + NH3 | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyuC | gene name, from the hyu gene cluster containing four genes with novel cluster organization characteristics | Siminovitchia fordii |
| L-carbamoylase | - |
Siminovitchia fordii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
assay at | Siminovitchia fordii |
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Release 2023.1 (January 2023)
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