Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion. X-ray data are collected to a resolution of 2.75 A. The crystals belong to space group P21212, with unit-cell parameters a = 103.2, b = 211.7, c = 43.1 A and two subunits in the asymmetric unit | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
SDS-PAGE, very similar to that deduced from its amino acid sequence (about 45 kDa) | Geobacillus stearothermophilus |
45000 | - |
2 * 45000, SDS-PAGE | Geobacillus stearothermophilus |
90000 | - |
gel filtration, SECHPLC of purified BsLcar | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
CECT43 | - |
Geobacillus stearothermophilus CECT43 | - |
CECT43 | - |
Purification (Comment) | Organism |
---|---|
purified to over 95% purity | Geobacillus stearothermophilus |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
BsLcar | - |
Geobacillus stearothermophilus |
L-N-carbamoylase | - |
Geobacillus stearothermophilus |
N-carbamoyl-L-amino-acid amidohydrolase | - |
Geobacillus stearothermophilus |