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Literature summary for 3.5.1.87 extracted from

  • Ishikawa, T.; Mukohara, Y.; Watabe, K.; Kobayashi, S.; Nakamura, H.
    Microbial conversion of DL-5-substituted hydantoins to the corresponding L-amino acids by Bacillus stearothermophilus NS1122A (1994), Biosci. Biotechnol. Biochem., 58, 265-270.
No PubMed abstract available
Search for more literature for 3.5.1.87

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.5 mM, strong activation, no activity of partially purified enzyme without Co2+, Mn2+ or Ni2+ Geobacillus stearothermophilus
Mn2+ 0.5 mM, strong activation, no activity of partially purified enzyme without Co2+, Mn2+ or Ni2+ Geobacillus stearothermophilus
Ni2+ 5.0 mM, strong activation, no activity of partially purified enzyme without Co2+, Mn2+ or Ni2+ Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus NS1122A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, phenyl-Sepharose CL-4B, partially purified Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-DL-alanine + H2O
-
 Geobacillus stearothermophilus L-alanine + CO2 + NH3
-
 ?
N-carbamoyl-DL-phenylalanine + H2O 25% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-phenylalanine + CO2 + NH3
-
 ?
N-carbamoyl-DL-serine + H2O 86% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-serine + CO2 + NH3
-
 ?
N-carbamoyl-DL-threonine + H2O 94% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-threonine + CO2 + NH3
-
 ?
N-carbamoyl-DL-valine + H2O
-
 Geobacillus stearothermophilus L-valine + CO2 + NH3
-
 ?
N-carbamoyl-glycine + H2O 71% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus glycine + CO2 + NH3
-
 ?
N-carbamoyl-L-asparagine + H2O 52% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-asparagine + CO2 + NH3
-
 ?
N-carbamoyl-L-glutamic acid + H2O 56% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-glutamic acid + CO2 + NH3
-
 ?
N-carbamoyl-L-isoleucine + H2O 55% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-isoleucine + CO2 + NH3
-
 ?
N-carbamoyl-L-leucine + H2O 9.1% of activity with N-carbamoyl-L-alanine Geobacillus stearothermophilus L-leucine + CO2 + NH3
-
 ?
N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Geobacillus stearothermophilus L-methionine + CO2 + NH3
-
 ?
N-carbamoyl-L-tyrosine + H2O 3% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-tyrosine + CO2 + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Geobacillus stearothermophilus