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Literature summary for 3.5.1.86 extracted from

  • McLeish, M.J.; Kneen, M.M.; Gopalakrishna, K.N.; Koo, C.W.; Babbitt, P.C.; Gerlt, J.A.; Kenyon, G.L.
    Identification and characterization of a mandelamide hydrolase and an NAD(P)+dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633 (2003), J. Bacteriol., 185, 2451-2456.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0198
-
 (S)-(+)-mandelamide at 30°C and pH 7.8 Pseudomonas putida
0.0342
-
 (R)-(+)-mandelamide at 30°C and pH 7.8 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52600
-
 sedimentation analysis Pseudomonas putida
53820
-
 electrospray mass spectrometry Pseudomonas putida
53820
-
 deduced from amino acid sequence Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 strain ATCC 12633
-

Purification (Commentary)

Purification (Comment) Organism
Sephacryl S-200 gel filtration, HiPrep Q anion-exchange column chromatography, and HiTrap Q column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(+)-mandelamide + H2O
-
 Pseudomonas putida (R)-(+)-mandelate + NH3
-
 ?
(S)-(+)-mandelamide + H2O
-
 Pseudomonas putida (S)-(+)-mandelate + NH3
-
 ?
3,4-dichloroisocoumarin + H2O
-
 Pseudomonas putida ?
-
 ?
3-phenylpropionamide + H2O
-
 Pseudomonas putida 3-phenylpropionate + NH3
-
 ?
additional information benzamide is not a substrate Pseudomonas putida ?
-
 ?
phenylacetamide + H2O
-
 Pseudomonas putida phenylacetate + NH3
-
 ?
phenylmethylsulfonyl fluoride + H2O
-
 Pseudomonas putida ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52600, sedimentation analysis Pseudomonas putida

Synonyms

Synonyms Comment Organism
MAH
-
 Pseudomonas putida
mandelamide hydrolase
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031 0.51 (S)-(+)-mandelamide at 30°C and pH 7.8 Pseudomonas putida
7.73
-
 (S)-(+)-mandelamide at 30°C and pH 7.8 Pseudomonas putida
16.1
-
 (R)-(+)-mandelamide at 30°C and pH 7.8 Pseudomonas putida