Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(S)-N-acetyl-1-phenylethylamine | activation and induction, addition of more than 3 g/l (S)-N-acetyl-1-phenylethylamine to medium affects growth and acylase production negatively | Rhodococcus equi |
Application | Comment | Organism |
---|---|---|
synthesis | economic synthesis of enantiomerically pure (S)-1-phenylethylamine. Enantiomerically pure chiral amines, (S)-enantiomer of 1-phenylethylamine is an example of such compound of high interest, a field of high interest for pharmaceutical industry | Rhodococcus equi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM: 5% inhibition | Rhodococcus equi | |
Ca2+ | 1 mM: 10% inhibition | Rhodococcus equi | |
Cd2+ | 1 mM: 15% inhibition | Rhodococcus equi | |
Co2+ | 1 mM: 31% inhibition | Rhodococcus equi | |
Cu2+ | 1 mM: 12% inhibition | Rhodococcus equi | |
Mg2+ | 1 mM: 10% inhibition | Rhodococcus equi | |
Mn2+ | 1 mM: 13% inhibition | Rhodococcus equi | |
additional information | no inhibition by 10 mM chelating agents: EDTA, citrate and 10 mM thiol-reducing agents: dithiothreitol, mercaptoethanol, reduced glutathione; no inhibition by 1 mM Fe3+ | Rhodococcus equi | |
Ni2+ | 1 mM: 60% inhibition | Rhodococcus equi | |
Pb2+ | 1 mM: 5% inhibition | Rhodococcus equi | |
phenylmethylsulfonyl fluoride | strong inhibition, 0.1 mM: 95% inhibition, 0.01 mM: 84% inhibition | Rhodococcus equi | |
V3+ | 1 mM: 11% inhibition | Rhodococcus equi | |
Zn2+ | 1 mM: 32% inhibition | Rhodococcus equi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
(S)-N-acetyl-1-phenylethylamine | - |
Rhodococcus equi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal cations not required | Rhodococcus equi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, two identical subunits, SDS-PAGE | Rhodococcus equi |
94000 | - |
gel filtration | Rhodococcus equi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus equi | - |
- |
- |
Rhodococcus equi Ac6 / DSM 10278 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus equi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-acetylphenylethylamine + H2O = phenylethylamine + acetate | induction of (S)-N-acetyl-1-phenylethylamine hydrolase | Rhodococcus equi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.87 | - |
- |
Rhodococcus equi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-N-acetyl-1-phenylethylamine + H2O | high enantioselectivity, enantioselectivity of enzyme is 350 for (S)-N-acetyl-1-phenylethylamine | Rhodococcus equi | (S)-1-phenylethylamine + acetate | enantiomerically pure (S)-1-phenylethylamine | ? | |
(S)-N-acetyl-1-phenylethylamine + H2O | substrate and inducer of enzyme | Rhodococcus equi | (S)-1-phenylethylamine + acetate | enantiomerically pure (S)-1-phenylethylamine | ? | |
(S)-N-acetyl-1-phenylethylamine + H2O | high enantioselectivity, enantioselectivity of enzyme is 350 for (S)-N-acetyl-1-phenylethylamine | Rhodococcus equi Ac6 / DSM 10278 | (S)-1-phenylethylamine + acetate | enantiomerically pure (S)-1-phenylethylamine | ? | |
(S)-N-acetyl-1-phenylethylamine + H2O | substrate and inducer of enzyme | Rhodococcus equi Ac6 / DSM 10278 | (S)-1-phenylethylamine + acetate | enantiomerically pure (S)-1-phenylethylamine | ? | |
additional information | enzyme is highly enantioselective | Rhodococcus equi | ? | - |
? | |
additional information | some related N-acetylated compounds are hydrolysed with variable enantiomeric selectivities | Rhodococcus equi | ? | - |
? | |
additional information | substrate specificity and enantioselectivity | Rhodococcus equi | ? | - |
? | |
additional information | enzyme is highly enantioselective | Rhodococcus equi Ac6 / DSM 10278 | ? | - |
? | |
additional information | some related N-acetylated compounds are hydrolysed with variable enantiomeric selectivities | Rhodococcus equi Ac6 / DSM 10278 | ? | - |
? | |
additional information | substrate specificity and enantioselectivity | Rhodococcus equi Ac6 / DSM 10278 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, two identical subunits, SDS-PAGE | Rhodococcus equi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodococcus equi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus equi |
30 | - |
at 30°C, pH 7.0, half-life of around 350 days. 30°C for 34 days: 94% of activity retained | Rhodococcus equi |
44 | - |
half-life: 10 min | Rhodococcus equi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI at pH 3.5 | Rhodococcus equi |
6.5 | 7 | broad pH optimum | Rhodococcus equi |
7 | - |
assay at | Rhodococcus equi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus equi |
5.5 | 9 | activity between | Rhodococcus equi |
6 | 8.5 | broad activity optimum between, activity lost rather quickly below pH 6.0 and above pH 8.5 with the decay being steeper on the acidic side of optimum curve | Rhodococcus equi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | stable between, activity lost rather quickly below pH 6.0 and above pH 8.5 with the decay being steeper on the acidic side of optimum curve | Rhodococcus equi |
7 | - |
at pH 7.0, 30°C, half-life of around 350 days | Rhodococcus equi |