Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | rapid inactivation even at an alkaline pH, 9.3 | Pseudomonas sp. | |
Cu2+ | - |
Pseudomonas sp. | |
EDTA | reactivation: Zn2+, 66%, Co2+, 40%, or Mn2+ 9% | Pseudomonas sp. | |
Fe2+ | - |
Pseudomonas sp. | |
Zn2+ | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | contains 2.06-2.61 gatom of Zn per mol of enzyme. The zinc atom is required for the catalytic activity and stability | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Pseudomonas sp. |
55000 | - |
1 * 55000, SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. 5f-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Acetyl-D-Glu + H2O | - |
Pseudomonas sp. | acetate + D-Glu | - |
? | |
N-Acetyl-D-Glu + H2O | - |
Pseudomonas sp. 5f-1 | acetate + D-Glu | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Zn2+ increases heat stability. Thermal stability increases about 10°C in the presence of Zn2+ or Co2+. The optimal concentration for Zn2+ at 45°C and pH 6.5 is 0.03-0.05 mM. The optimal concentration for Co2+ at 45°C, pH 6.5 is 1.0-2.0 mM | Pseudomonas sp. |
45 | - |
10 min, in absence of added metal ions, 60-70% loss of activity | Pseudomonas sp. |