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Literature summary for 3.5.1.81 extracted from
- Cloning, expression and characterization of D-aminoacylase from Achromobacter xylosoxidans subsp. denitrificans ATCC 15173 (2013), Microbiol. Res., 168, 360-366.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme can be useful for industrial production of D-amino acids | Achromobacter denitrificans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ADdan, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) using pET-28a with a T7 promoter | Achromobacter denitrificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | low inhibition | Achromobacter denitrificans |  |
| Cu2+ | - |
Achromobacter denitrificans | |
| Fe2+ | - |
Achromobacter denitrificans | |
| Hg2+ | - |
Achromobacter denitrificans | |
| Mn2+ | low inhibition | Achromobacter denitrificans | |
| Ni2+ | low inhibition | Achromobacter denitrificans | |
| Zn2+ | - |
Achromobacter denitrificans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Mg2+ has no significant influence on the recombinant D-aminoacylase | Achromobacter denitrificans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, recombinant His-tagged enzyme, SDS-PAGE | Achromobacter denitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acyl-D-amino acid + H2O | Achromobacter denitrificans | - |
a carboxylate + D-amino acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter denitrificans | S4T8N6 | gene ADdan | - |
| Achromobacter denitrificans ATCC 15173 | S4T8N6 | gene ADdan | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 8.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chhromatography and ultrafiltration to homogeneity | Achromobacter denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 204 | - |
purified recombinant enzyme, pH 7.8, 37°C | Achromobacter denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-alanine + H2O | 16% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans | acetate + D-alanine | - |
? | |
| N-acetyl-D-aspartate + H2O | 25% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans | acetate + D-aspartate | - |
? | |
| N-acetyl-D-aspartate + H2O | 25% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans ATCC 15173 | acetate + D-aspartate | - |
? | |
| N-acetyl-D-leucine + H2O | 61% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans | acetate + D-leucine | - |
? | |
| N-acetyl-D-leucine + H2O | 61% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans ATCC 15173 | acetate + D-leucine | - |
? | |
| N-acetyl-D-methionine + H2O | best substrate | Achromobacter denitrificans | acetate + D-methionine | - |
? | |
| N-acetyl-D-methionine + H2O | best substrate | Achromobacter denitrificans ATCC 15173 | acetate + D-methionine | - |
? | |
| N-acetyl-D-phenylalanine + H2O | 54% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans | acetate + D-phenylalanine | - |
? | |
| N-acetyl-D-phenylalanine + H2O | 54% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans ATCC 15173 | acetate + D-phenylalanine | - |
? | |
| N-acetyl-D-tryptophan + H2O | 14% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans | acetate + D-tryptophan | - |
? | |
| N-acetyl-D-tyrosine + H2O | 5.0% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans | acetate + D-tyrosine | - |
? | |
| N-acetyl-D-valine + H2O | 8.0% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans | acetate + D-valine | - |
? | |
| N-acetyl-D-valine + H2O | 8.0% of the activity with N-acetyl-D-methionine | Achromobacter denitrificans ATCC 15173 | acetate + D-valine | - |
? | |
| N-acyl-D-amino acid + H2O | - |
Achromobacter denitrificans | a carboxylate + D-amino acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, recombinant His-tagged enzyme, SDS-PAGE | Achromobacter denitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-Aminoacylase | - |
Achromobacter denitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme | Achromobacter denitrificans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | activity range, recombinant enzyme, the activity decreased rapidly above 50°C | Achromobacter denitrificans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
purified recombinant enzyme, pH 7.5, 30 min, 80% activity remaining | Achromobacter denitrificans |
| 50 | - |
purified recombinant enzyme, pH 7.5, 30 min, 50% activity remaining | Achromobacter denitrificans |
| 55 | - |
purified recombinant enzyme, pH 7.5, 30 min, inactivation | Achromobacter denitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Achromobacter denitrificans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | purified recombinant enzyme, 37°C, 30 min, stable at | Achromobacter denitrificans |
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