Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not inducible by N-acyl-D-amino acids and D-amino acid | Trichoderma sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.89 | - |
N-acetyl-D-methionine | pH 8.0, 50°C | Trichoderma sp. | |
2.04 | - |
N-acetyl-D-leucine | pH 8.0, 50°C | Trichoderma sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Trichoderma sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma sp. | - |
isolated from soil, constitutive enzyme | - |
Trichoderma sp. SKW-36 | - |
isolated from soil, constitutive enzyme | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration | Trichoderma sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | constitutive enzyme | Trichoderma sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42.1 | - |
purified enzyme | Trichoderma sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-leucine + H2O | - |
Trichoderma sp. | acetate + D-leucine | - |
? | |
N-acetyl-D-leucine + H2O | - |
Trichoderma sp. SKW-36 | acetate + D-leucine | - |
? | |
N-acetyl-D-methionine + H2O | - |
Trichoderma sp. | acetate + D-methionine | - |
? | |
N-acetyl-D-methionine + H2O | - |
Trichoderma sp. SKW-36 | acetate + D-methionine | - |
? | |
N-acetyl-D-phenylalanine + H2O | - |
Trichoderma sp. | acetate + D-phenylalanine | - |
? | |
N-acetyl-D-phenylalanine + H2O | - |
Trichoderma sp. SKW-36 | acetate + D-phenylalanine | - |
? | |
N-acetyl-D-valine + H2O | - |
Trichoderma sp. | acetate + D-valine | - |
? | |
N-acetyl-D-valine + H2O | - |
Trichoderma sp. SKW-36 | acetate + D-valine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Trichoderma sp. |
Synonyms | Comment | Organism |
---|---|---|
D-Aminoacylase | - |
Trichoderma sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trichoderma sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, fully stable up to 40°C | Trichoderma sp. |
60 | - |
purified enzyme, inactivation | Trichoderma sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Trichoderma sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | purified enzyme, fully stable | Trichoderma sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trichoderma sp. | - |
- |
4.5 |