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Literature summary for 3.5.1.81 extracted from

  • Tsai, Y.C.; Lin, C.S.; Tseng, T.H.; Lee, H.; Wang, Y.J.
    Production and immobilization of D-aminoacylase of Alcaligenes faecalis DA1 for optimal resolution of N-acyl-DL-amino acids (1992), Enzyme Microb. Technol., 14, 384-389.
    View publication on PubMed
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General Stability

General Stability Organism
immobilized enzyme retains 90% of the initial activity after 17 days of continous operation Alcaligenes faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 N-Acetyl-D-Met immobilized enzyme Alcaligenes faecalis
1
-
 N-Acetyl-D-Met soluble enzyme Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis DA1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Alcaligenes faecalis

Storage Stability

Storage Stability Organism
4°C, 0.02% NaN3, immobilized enzyme is stable for more than 2 months Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-Ala + H2O
-
 Alcaligenes faecalis acetate + D-Ala
-
 ?
N-Acetyl-D-Asn + H2O
-
 Alcaligenes faecalis acetate + D-Asn
-
 ?
N-acetyl-D-Leu + H2O
-
 Alcaligenes faecalis acetate + D-Leu
-
 ?
N-acetyl-D-Leu + H2O
-
 Alcaligenes faecalis DA1 acetate + D-Leu
-
 ?
N-Acetyl-D-Met + H2O
-
 Alcaligenes faecalis acetate + D-Met
-
 ?
N-Acetyl-D-Met + H2O
-
 Alcaligenes faecalis DA1 acetate + D-Met
-
 ?
N-Acetyl-D-Phe + H2O
-
 Alcaligenes faecalis acetate + D-Phe
-
 ?
N-Acetyl-D-Phe + H2O
-
 Alcaligenes faecalis DA1 acetate + D-Phe
-
 ?
N-Acetyl-D-phenylglycine + H2O
-
 Alcaligenes faecalis acetate + D-phenylglycine
-
 ?
N-Acetyl-D-phenylglycine + H2O
-
 Alcaligenes faecalis DA1 acetate + D-phenylglycine
-
 ?
N-Acetyl-D-Trp + H2O
-
 Alcaligenes faecalis acetate + D-Trp
-
 ?
N-Acetyl-D-Trp + H2O
-
 Alcaligenes faecalis DA1 acetate + D-Trp
-
 ?
N-Chloroacetyl-D-Val + H2O
-
 Alcaligenes faecalis Chloroacetate + D-Val
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 soluble and immobilized enzyme Alcaligenes faecalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 half-life of the immobilized enzyme: 18 days Alcaligenes faecalis
30
-
 protein concentration 0.7 mg/ml, half-life of the free enzyme: 4 days Alcaligenes faecalis
40
-
 soluble and immobilized enzyme, stable up to Alcaligenes faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 soluble and immobilized enzyme Alcaligenes faecalis

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6: about 60% of maximal activity, pH 10: about 30% of maximal activity Alcaligenes faecalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 stable between pH 5.0 and pH 11.0, soluble enzyme Alcaligenes faecalis
5 9 stable between pH 5 and pH 9, immobilized enzyme Alcaligenes faecalis