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Literature summary for 3.5.1.81 extracted from

  • Wakayama, M.; Katsuno, Y.; Hayashi, S.; Miyamoto, Y.; Sakai, K.; Moriguchi, M.
    Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli (1995), Biosci. Biotechnol. Biochem., 59, 2115-2119.
    View publication on PubMed
Search for more literature for 3.5.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Achromobacter xylosoxidans

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM Achromobacter xylosoxidans
Cu2+ 1 mM, 98% inhibition Achromobacter xylosoxidans
Fe2+ 1 mM Achromobacter xylosoxidans
Ni2+ 1 mM Achromobacter xylosoxidans
Zn2+ 1 mM, 94% inhibition Achromobacter xylosoxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Achromobacter xylosoxidans
51918
-
 x * 51918, calculation from nucleotide sequence Achromobacter xylosoxidans
52000
-
 x * 52000, SDS-PAGE Achromobacter xylosoxidans

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans
-
 subsp. xylosoxydans A-6
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1000
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-Leu + H2O
-
 Achromobacter xylosoxidans acetate + D-Leu
-
 ?
N-Acetyl-D-Met + H2O
-
 Achromobacter xylosoxidans acetate + D-Met
-
 ?
N-Acetyl-D-norleucine + H2O
-
 Achromobacter xylosoxidans acetate + D-norleucine
-
 ?
N-Acetyl-D-Phe + H2O
-
 Achromobacter xylosoxidans acetate + D-Phe
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Achromobacter xylosoxidans
? x * 51918, calculation from nucleotide sequence Achromobacter xylosoxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Achromobacter xylosoxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Achromobacter xylosoxidans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 optimal stability between pH 7 and pH 8 Achromobacter xylosoxidans