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Literature summary for 3.5.1.79 extracted from

  • Black, T.D.; Briggs, B.S.; Evans, R.; Muth, W.L.; Vangala, S.; Zmijewski, M.J.
    o-Phthalyl amidase in the synthesis of loracarbef: process development using this novel biocatalyst (1996), Biotechnol. Lett., 18, 875-880.
No PubMed abstract available
Search for more literature for 3.5.1.79

Application

Application Comment Organism
synthesis alternative chemical route in removing the phthalimido protecting group Xanthobacter agilis

General Stability

General Stability Organism
pH-stability and temperature-stability is improved significantly by increasing ionic strength of the buffer Xanthobacter agilis
the enzyme stability is optimal at salt concentrations of 0.2 M and above Xanthobacter agilis
the substrate phthalimido carbacephem stablilizes the enzyme Xanthobacter agilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 1 * 49000, SDS-PAGE Xanthobacter agilis
49724
-
 1 * 49724, calculation from nucleotide sequence Xanthobacter agilis

Organism

Organism UniProt Comment Textmining
Xanthobacter agilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large scale Xanthobacter agilis

Reaction

Reaction Comment Organism Reaction ID
A phthalylamide + H2O = phthalic acid + a substituted amine mechanism Xanthobacter agilis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9
-
-
 Xanthobacter agilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Carboxybenzoylamine-D-Phe-L-Ala
-
 Xanthobacter agilis ?
-
 ?
2-Carboxybenzoylamine-D-phenylglycine-methylester
-
 Xanthobacter agilis ?
-
 ?
2-Carboxybenzoylamine-L-Phe-L-Ala
-
 Xanthobacter agilis ?
-
 ?
additional information broad specificity for o-phthalylated amides, absolute requirement for the o-phthalyl protecting group Xanthobacter agilis ?
-
 ?
o-Phthalyl carbacephem
-
 Xanthobacter agilis Loracarbef + phthalic acid
-
 ?
[4-[(2-Carboxybenzoyl)amino]-1H-imidazol-1-yl]octanoic acid
-
 Xanthobacter agilis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Xanthobacter agilis
monomer 1 * 49724, calculation from nucleotide sequence Xanthobacter agilis