Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His6-tag | Crithidia fasciculata |
Protein Variants | Comment | Organism |
---|---|---|
C79A | complete loss of amidase activity without affecting synthetase activity | Crithidia fasciculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
spermidine | pH 7.3, synthetase activity | Crithidia fasciculata | |
0.114 | - |
ATP | pH 7.3, synthetase activity | Crithidia fasciculata | |
0.242 | - |
reduced glutathione | pH 7.3, synthetase activity | Crithidia fasciculata | |
0.5 | - |
glutathionylspermidine | pH 7.3, amidase activity | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
bifunctional glutathionylspermidine synthetase, EC 6.3.1.8, and amidase, EC 3.5.1.78 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathionylspermidine + H2O | - |
Crithidia fasciculata | glutathione + spermidine | - |
r | |
trypanothione + H2O | hydrolysis at 1.5% the rate of glutathionylspermidine | Crithidia fasciculata | glutathione + glutathionylspermidine | - |
? |