Literature summary for 3.5.1.77 extracted from

Jiang, S.; Li, C.; Zhang, W.; Cai, Y.; Yang, Y.; Yang, S.; Jiang, W.
Directed evolution and structural analysis of N-carbamoyl-D-amino acid amidohydrolase provide insights into recombinant protein solubility in Escherichia coli (2007), Biochem. J., 402, 429-437.

Search for more literature for 3.5.1.77

Application

Application	Comment	Organism
synthesis	important enzyme involved in semi-synthesis of beta-lactam antibiotics in industry	Ralstonia pickettii

Cloned(Commentary)

Cloned (Comment)	Organism
mutant A18T/Y30N/K34E is overexpressed in Escherichia coli and up to 80% of it is soluble	Ralstonia pickettii

Protein Variants

Protein Variants	Comment	Organism
A18T/Y30N/K34E	kinetic properties and thermodynamic parameters of the mutant enzyme are identical with those of the wild-type enzyme. More than 80% improve in solubility compared to wild-type enzyme	Ralstonia pickettii
K34D	mutant has higher solubility than wild-type enzyme	Ralstonia pickettii
K34E	mutant has higher solubility than wild-type enzyme	Ralstonia pickettii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.8	-	N-carbamoyl-D-p-hydroxyphenylglycine	mutant enzyme A18T/Y30N/K34E	Ralstonia pickettii
0.82	-	N-carbamoyl-D-p-hydroxyphenylglycine	wild-type enzyme	Ralstonia pickettii

Organism

Organism	UniProt	Comment	Textmining
Ralstonia pickettii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant mutant A18T/Y30N/K34E	Ralstonia pickettii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-carbamoyl-D-p-hydroxyphenylglycine + H2O	-	Ralstonia pickettii	D-p-hydroxyphenylglycine + NH3 + CO2	-	?

Synonyms

Synonyms	Comment	Organism
Dcase	-	Ralstonia pickettii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.4	-	N-carbamoyl-D-p-hydroxyphenylglycine	wild-type enzyme	Ralstonia pickettii
5.9	-	N-carbamoyl-D-p-hydroxyphenylglycine	mutant enzyme A18T/Y30N/K34E	Ralstonia pickettii

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Release 2023.1 (January 2023)