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Literature summary for 3.5.1.77 extracted from

  • Grifantini, R.; Pratesi, C.; Galli, G.; Grandi, G.
    Topological mapping of the cysteine residues of N-carbamoyl-D-amino-acid amidohydrolase and their role in enzymatic activity (1996), J. Biol. Chem., 271, 9326-9331.
    View publication on PubMed
Search for more literature for 3.5.1.77

Application

Application Comment Organism
synthesis enzyme is used for the industrial production of D-amino acids Agrobacterium tumefaciens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
C172A mutant enzyme Cys172Ala or Cys172Ser is completely inactive. Substitution of any of the other Cys has no effect on enzyme activity Agrobacterium tumefaciens
C172S mutant enzyme Cys172Ala or Cys172Ser is completely inactive. Substitution of any of the other Cys has no effect on enzyme activity Agrobacterium tumefaciens

General Stability

General Stability Organism
stable in absence rather than in presence of 2-mercaptoethanol Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate
-
 Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Agrobacterium tumefaciens
34000
-
 2 * 34000, calculation from nucleotide sequence Agrobacterium tumefaciens
67000
-
 gel filtration Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens NTRRL B11291
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.1
-
-
 Agrobacterium tumefaciens

Storage Stability

Storage Stability Organism
4°C, phosphate buffer, progressive loss of activity Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium tumefaciens D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?
N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium tumefaciens NTRRL B11291 D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Agrobacterium tumefaciens
dimer 2 * 34000, calculation from nucleotide sequence Agrobacterium tumefaciens