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Literature summary for 3.5.1.76 extracted from

  • Shimizu, S.; Ogawa, J.; Chung, M.C.M.; Yamada, H.
    Purification and characterization of a novel enzyme, arylalkyl acylamidase, from Pseudomonas putida Sc2 (1992), Eur. J. Biochem., 209, 375-382.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Pseudomonas putida
2,2'-dipyridyl
-
Pseudomonas putida
5,5'-dithiobis(2-nitrobenzoic acid) weak Pseudomonas putida
Ag2SO4
-
Pseudomonas putida
HgCl2
-
Pseudomonas putida
iodoacetate weak Pseudomonas putida
N-bromosuccinimide
-
Pseudomonas putida
NEM
-
Pseudomonas putida
PCMB
-
Pseudomonas putida
phenylhydrazine
-
Pseudomonas putida
Semicarbazide
-
Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
N-acetyl-2-phenylethylamine
-
Pseudomonas putida
1.5
-
Melatonin
-
Pseudomonas putida
0.34
-
N-acetylserotonin
-
Pseudomonas putida
1.6
-
N-Acetyl-3-phenylpropylamine
-
Pseudomonas putida
5
-
N-acetyldopamine
-
Pseudomonas putida
8.9
-
(R)-N-acetyl-1-methyl-3-phenylpropylamine
-
Pseudomonas putida
41
-
N-Acetylbenzylamine
-
Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas putida
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 activates Pseudomonas putida
CuSO4 activates Pseudomonas putida
MnCl2 activates Pseudomonas putida
CoCl2 activates Pseudomonas putida
ZnSO4 activates Pseudomonas putida
NiCl2 activates Pseudomonas putida
CdCl2 activates Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
gel filtration Pseudomonas putida
42000
-
4 * 42000, SDS-PAGE Pseudomonas putida
42630
-
4 * 42630, SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida Sc2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.25
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with acetanilide derivatives Pseudomonas putida ?
-
?
additional information no activity with acetanilide derivatives Pseudomonas putida Sc2 ?
-
?
N-Acetyl-2-phenylethylamine + H2O
-
Pseudomonas putida 2-Phenylethylamine + acetate
-
?
N-Acetyl-2-phenylethylamine + H2O
-
Pseudomonas putida Sc2 2-Phenylethylamine + acetate
-
?
N-Acetyl-1-methyl-3-phenylpropylamine + H2O (R)-isomer Pseudomonas putida (S)-1-Methyl-3-phenylpropylamine + acetate
-
?
N-Acetyl-1-methyl-3-phenylpropylamine + H2O (R)-isomer Pseudomonas putida Sc2 (S)-1-Methyl-3-phenylpropylamine + acetate
-
?
N-Acetylbenzylamine + H2O
-
Pseudomonas putida ?
-
?
N-Acetylbenzylamine + H2O
-
Pseudomonas putida Sc2 ?
-
?
N-Acetyl-3-phenylpropylamine
-
Pseudomonas putida ?
-
?
N-Acetyl-3-phenylpropylamine
-
Pseudomonas putida Sc2 ?
-
?
N-acetyldopamine + H2O
-
Pseudomonas putida ?
-
?
N-Acetylserotonin + H2O
-
Pseudomonas putida ?
-
?
Melatonin + H2O
-
Pseudomonas putida ?
-
?
Acetic acid 2-phenylethyl ester + H2O
-
Pseudomonas putida ?
-
?
Acetic acid 3-phenylpropyl ester + H2O
-
Pseudomonas putida ?
-
?
(R)-N-acetyl-1-methyl-3-phenylpropylamine + H2O
-
Pseudomonas putida (S)-1-methyl-3-phenylpropylamine + acetate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 42000, SDS-PAGE Pseudomonas putida
tetramer 4 * 42630, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 75 55°C: about 45% of maximal activity, 75°C: about 20% of maximal activity Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 7.0, stable below Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
with N-acetyl-2-phenylethylamine as substrate Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 50% of maximal activity, pH 10: about 40% of maximal activity, with N-acetyl-2-phenylethalamine as substrate Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
30°C, 30 min, most stable under alkaline conditions Pseudomonas putida