Cloned (Comment) | Organism |
---|---|
gene ureC, functional recombinant expression of His-tagged enzyme from operon ureABC in Escherichia coli strain BL21(DE3) in inclusion bodies | Providencia rettgeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanol | 50% inhibition at 30% v/v | Providencia rettgeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Providencia rettgeri | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | ? | - |
? | |
additional information | Providencia rettgeri JN-B815 | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | ? | - |
? | |
additional information | Providencia rettgeri CGMCC 8326 | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | ? | - |
? | |
urethane + H2O | Providencia rettgeri | - |
ethanol + CO2 + NH3 | - |
? | |
urethane + H2O | Providencia rettgeri JN-B815 | - |
ethanol + CO2 + NH3 | - |
? | |
urethane + H2O | Providencia rettgeri CGMCC 8326 | - |
ethanol + CO2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Providencia rettgeri | - |
- |
- |
Providencia rettgeri CGMCC 8326 | - |
- |
- |
Providencia rettgeri JN-B815 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme, refolded from Escherichia coli strain BL21(DE3) inclusion bodies, 1.22fold by nickel affinity chromatography | Providencia rettgeri |
Renatured (Comment) | Organism |
---|---|
recombinant His-tagged enzyme is solubilized and refolded from Escherichia coli strain BL21(DE3) inclusion bodies. Secondary structure analysis of renatured recombinant urease | Providencia rettgeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.8 | - |
purified recombinant apoenzyme, urethanase activity, pH 4.5, temperature not specified in the publication | Providencia rettgeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | Providencia rettgeri | ? | - |
? | |
additional information | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | Providencia rettgeri JN-B815 | ? | - |
? | |
additional information | the enzyme exhibits not only urease activity (cf. acid urease, EC 3.5.1.5), but also urethanase activity | Providencia rettgeri CGMCC 8326 | ? | - |
? | |
urethane + H2O | - |
Providencia rettgeri | ethanol + CO2 + NH3 | - |
? | |
urethane + H2O | - |
Providencia rettgeri JN-B815 | ethanol + CO2 + NH3 | - |
? | |
urethane + H2O | - |
Providencia rettgeri CGMCC 8326 | ethanol + CO2 + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | also see for EC 3.5.1.5 | Providencia rettgeri |
UreC | - |
Providencia rettgeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Providencia rettgeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
recombinant enzyme | Providencia rettgeri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8.5 | activity range, recombinant enzyme, profile overview | Providencia rettgeri |