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Literature summary for 3.5.1.75 extracted from
- Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions (2015), Anal. Methods, 7, 1261-1264 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is used in spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions, method, overview. Detection of ethyl carbamate (urethane) in Chinese rice wine. Urethane is known as a genotoxic carcinogen1 that widely exists in fermented foods and alcoholic beverages, such as bread, yogurt, cheese, brandy, Chinese rice wine, sake, and wine, due to the natural biochemical processes in the fermentation process | Talaromyces variabilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urethane + H2O | Talaromyces variabilis | - |
ethanol + CO2 + NH3 | - |
? |  |
| urethane + H2O | Talaromyces variabilis JN-A525 | - |
ethanol + CO2 + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces variabilis | - |
- |
- |
| Talaromyces variabilis JN-A525 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | a highly sensitive spectrophotometric method for ethyl carbamate (EC) determination is established through glutamate dehydrogenase/urethanase cascade reactions and the corresponding change in NADH concentration. The absorbance at 340 nm is linearly related to the EC concentration within the range of 300-5000 nM, with a low detection limit of 9.28 nM. Method optimization. The absorbance declines slowly at either pH 4.5 or pH 8.0, which is around the optimal pH of one of the enzymes. But the absorbance decreases with the fastest rate at pH 6.0 | Talaromyces variabilis | ? | - |
? | |
| additional information | a highly sensitive spectrophotometric method for ethyl carbamate (EC) determination is established through glutamate dehydrogenase/urethanase cascade reactions and the corresponding change in NADH concentration. The absorbance at 340 nm is linearly related to the EC concentration within the range of 300-5000 nM, with a low detection limit of 9.28 nM. Method optimization. The absorbance declines slowly at either pH 4.5 or pH 8.0, which is around the optimal pH of one of the enzymes. But the absorbance decreases with the fastest rate at pH 6.0 | Talaromyces variabilis JN-A525 | ? | - |
? | |
| urethane + H2O | - |
Talaromyces variabilis | ethanol + CO2 + NH3 | - |
? | |
| urethane + H2O | - |
Talaromyces variabilis JN-A525 | ethanol + CO2 + NH3 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Talaromyces variabilis |
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Release 2023.1 (January 2023)
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