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Literature summary for 3.5.1.75 extracted from
- Purification and characterization of a urethanase from Penicillium variabile (2014), Appl. Biochem. Biotechnol., 172, 351-360.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | with good ethanol tolerance, the crude urethanase is able to reduce ethyl carbamate, i.e. urethane, in Chinese rice wine without the change of flavor substance in wine | Talaromyces variabilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0272 | - |
urethane | pH 6.0, 50°C | Talaromyces variabilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13700 | - |
x * 13700, SDS-PAGE | Talaromyces variabilis |
| 96000 | - |
gel filtration | Talaromyces variabilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urethane + H2O | Talaromyces variabilis | - |
ethanol + CO2 + NH3 | - |
? | |
| urethane + H2O | Talaromyces variabilis JN-A525 | - |
ethanol + CO2 + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces variabilis | - |
- |
- |
| Talaromyces variabilis JN-A525 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ultrasonication, concentration by PEG 20000, gel filtration, and ultrafilatration | Talaromyces variabilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urethane + H2O | - |
Talaromyces variabilis | ethanol + CO2 + NH3 | - |
? | |
| urethane + H2O | - |
Talaromyces variabilis JN-A525 | ethanol + CO2 + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | peptide map fingerprinting analysis by MALDI/TOF-TOF mass spectrometry, the N-terminal sequence of urethanase is GTNTADNDAA | Talaromyces variabilis |
| oligomer | x * 13700, SDS-PAGE | Talaromyces variabilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Talaromyces variabilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | purified native enzyme, stable at | Talaromyces variabilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Talaromyces variabilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | purified native enzyme, stable at | Talaromyces variabilis |
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