Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is strongly induced by acetanilide, effect of carbon sources and inducers on cell growth, overview | Rhodococcus equi |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Rhodococcus equi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 37% inhibition at 1 mM | Rhodococcus equi | |
Cd2+ | 18% inhibition at 1 mM | Rhodococcus equi | |
Co2+ | 11% inhibition at 1 mM | Rhodococcus equi | |
Cu2+ | 61% inhibition at 1 mM | Rhodococcus equi | |
Hg2+ | 98% inhibition at 1 mM | Rhodococcus equi | |
additional information | no or poor inhibition by Mn2+, EDTA, 8-hydroxyquinoline, DTT, and iodoacetamide | Rhodococcus equi | |
Ni2+ | 32% inhibition at 1 mM | Rhodococcus equi | |
PMSF | 84% inhibition at 1 mM | Rhodococcus equi | |
Zn2+ | 21% inhibition at 1 mM | Rhodococcus equi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
4-nitrophenyl acetate | pH 7.0, 30°C | Rhodococcus equi | |
0.07 | - |
ethyl N-phenylcarbamate | pH 7.0, 30°C | Rhodococcus equi | |
0.1 | - |
4-nitrophenylbutyrate | pH 7.0, 30°C | Rhodococcus equi | |
0.13 | - |
acetanilide | pH 7.0, 30°C | Rhodococcus equi | |
0.15 | - |
4-nitroacetanilide | pH 7.0, 30°C | Rhodococcus equi | |
2.49 | - |
L-alanine 4-nitroanilide | pH 7.0, 30°C | Rhodococcus equi | |
2.5 | - |
butyramide | pH 7.0, 30°C | Rhodococcus equi | |
6.59 | - |
ethyl carbamate | pH 7.0, 30°C | Rhodococcus equi | |
9.4 | - |
phenyl acetamide | pH 7.0, 30°C | Rhodococcus equi | |
11.8 | - |
Benzamide | pH 7.0, 30°C | Rhodococcus equi | |
49 | - |
acetamide | pH 7.0, 30°C | Rhodococcus equi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 20% activation at 1 mM | Rhodococcus equi | |
NEM | 12% activation at 1 mM | Rhodococcus equi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
approximately, gel filtration | Rhodococcus equi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexamethylene dicarbamic acid dibutyl ester + H2O | Rhodococcus equi | - |
? | - |
? | |
methylene bisphenyl dicarbamic acid dibutyl ester + H2O | Rhodococcus equi | - |
? | - |
? | |
additional information | Rhodococcus equi | the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters | ? | - |
? | |
additional information | Rhodococcus equi TB-60 | the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters | ? | - |
? | |
toluene-2,4-dicarbamic acid dibutyl ester + H2O | Rhodococcus equi | - |
toluene diamine + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus equi | - |
isolated from mineral medium | - |
Rhodococcus equi TB-60 | - |
isolated from mineral medium | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography | Rhodococcus equi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | effect of carbon sources and inducers on cell growth, overview | Rhodococcus equi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus equi |
6 | - |
purified native enzyme | Rhodococcus equi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitroacetanilide + H2O | - |
Rhodococcus equi | ? | - |
? | |
4-nitrophenyl acetate + H2O | - |
Rhodococcus equi | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Rhodococcus equi TB-60 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Rhodococcus equi | ? | - |
? | |
acetamide + H2O | - |
Rhodococcus equi | ? | - |
? | |
acetamide + H2O | - |
Rhodococcus equi TB-60 | ? | - |
? | |
acetanilide + H2O | - |
Rhodococcus equi | ? | - |
? | |
benzamide + H2O | - |
Rhodococcus equi | ? | - |
? | |
benzamide + H2O | - |
Rhodococcus equi TB-60 | ? | - |
? | |
butyl carbamate + H2O | - |
Rhodococcus equi | CO2 + NH3 + butanol | - |
? | |
butyramide + H2O | - |
Rhodococcus equi | ? | - |
? | |
ethyl carbamate + H2O | - |
Rhodococcus equi | CO2 + NH3 + ethanol | - |
? | |
ethyl N-phenylcarbamate + H2O | - |
Rhodococcus equi | CO2 + aniline + ethanol | - |
? | |
hexamethylene dicarbamic acid dibutyl ester + H2O | - |
Rhodococcus equi | ? | - |
? | |
hexamethylene dicarbamic acid dibutyl ester + H2O | - |
Rhodococcus equi | ? | product identification and quantification, overview | ? | |
L-alanine-4-nitroanilide + H2O | - |
Rhodococcus equi | ? | - |
? | |
methylene bisphenyl dicarbamic acid dibutyl ester + H2O | - |
Rhodococcus equi | ? | - |
? | |
methylene bisphenyl dicarbamic acid dibutyl ester + H2O | - |
Rhodococcus equi | ? | product identification and quantification, overview | ? | |
additional information | the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters | Rhodococcus equi | ? | - |
? | |
additional information | substrate specificity, overview, no activity with L-leucine-4-nitroanilide, methyl benzoate, and ethyl benzoate | Rhodococcus equi | ? | - |
? | |
additional information | the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters | Rhodococcus equi TB-60 | ? | - |
? | |
additional information | substrate specificity, overview, no activity with L-leucine-4-nitroanilide, methyl benzoate, and ethyl benzoate | Rhodococcus equi TB-60 | ? | - |
? | |
phenyl acetamide + H2O | - |
Rhodococcus equi | ? | - |
? | |
toluene-2,4-dicarbamic acid dibutyl ester + H2O | - |
Rhodococcus equi | toluene diamine + ? | - |
? | |
toluene-2,4-dicarbamic acid dibutyl ester + H2O | - |
Rhodococcus equi | toluene diamine + ? | product identification and quantification, overview | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Rhodococcus equi |
Synonyms | Comment | Organism |
---|---|---|
urethane hydrolase | - |
Rhodococcus equi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rhodococcus equi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Rhodococcus equi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | rapid inactivation at pH 4.0, more slowly inactivation at 60°C | Rhodococcus equi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Rhodococcus equi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | stable | Rhodococcus equi |