Application | Comment | Organism |
---|---|---|
nutrition | enzyme may be practically applicable in removal of urethane from alcoholic beverages, because very high ethanol resistance, high activity at acidic condition, pH 5.0 and very low Km value for urethane | Micrococcus sp. |
nutrition | great advantage for industrial removal of urethane, potentially carcinogenic, mutagenic and teratogenic to human, from alcoholic beverages | Micrococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM: 41% inhibition, 20 mM: complete inhibition | Micrococcus sp. | |
Cu2+ | 1 mM: 37% inhibition, 20 mM: 69% inhibition | Micrococcus sp. | |
EDTA | no inhibition | Micrococcus sp. | |
Fe2+ | 1 mM: 67% inhibition, 10 mM: complete inhibition | Micrococcus sp. | |
iodoacetamide | 0.1 mM: 48% inhibition | Micrococcus sp. | |
iodoacetic acid | 0.1 mM: 40% inhibition | Micrococcus sp. | |
Mn2+ | slight inhibition | Micrococcus sp. | |
additional information | no inhibition by EDTA | Micrococcus sp. | |
Ni2+ | 1 mM: 14% inhibition, 20 mM: 58% inhibition | Micrococcus sp. | |
p-chloromercuribenzoate | 0.1 mM: 52% inhibition | Micrococcus sp. | |
phenylmethylsulfonyl fluoride | 0.1 mM: 61% inhibition; PMSF | Micrococcus sp. | |
Zn2+ | 1 mM: 29% inhibition, 25 mM: complete inhibition | Micrococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic properties | Micrococcus sp. | |
0.078 | - |
urethane | - |
Micrococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal ions not required | Micrococcus sp. | |
additional information | no effect of Ca2+, Mg2+, Na+, major cations of sea water | Micrococcus sp. |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 100% resistant to 10%, v/v, ethanol, above 10% ethanol the activity decreased depending upon ethanol concentration, resistant to 20%, v/v, ethanol: 82% activity remained | Micrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus sp. | - |
marine bacterium, associated with the marine sponge, Spirastrella species | - |
Purification (Comment) | Organism |
---|---|
partial | Micrococcus sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
urethane + H2O = ethanol + CO2 + NH3 | thermodynamic data | Micrococcus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | 80% of urethanase activity is cell associated | Micrococcus sp. | - |
additional information | 80% of urethanase activity is cell associated | Micrococcus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Micrococcus sp. | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Micrococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | activity between | Micrococcus sp. |
50 | - |
40% of maximum activity | Micrococcus sp. |
55 | - |
22% of maximum activity | Micrococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable up to, 1 h, stability sharply declined above | Micrococcus sp. |
50 | - |
half-life of enzyme at 50°C: 120 min | Micrococcus sp. |
60 | - |
half-life of enzyme at 60°C: 30 min | Micrococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
significantly higher urethanase activity at acidic condition than at neutral condition | Micrococcus sp. |
5 | - |
- |
Micrococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
urethanase activity sharply declines in alkaline region | Micrococcus sp. |
4 | 8.5 | half of maximum activity at pH 4.0 and pH 8.5 | Micrococcus sp. |
6 | 7 | pH 6.0: 98% of maximum activity, pH 7.0: 78% of maximum activity | Micrococcus sp. |