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Literature summary for 3.5.1.75 extracted from
- Urethane-hydrolyzing enzyme from Citrobacter sp (1990), Chem. Pharm. Bull., 38, 1326-1328.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | practically ineffective for the elimination of urethane from alcoholic beverages, because the enzyme is inactive in high concentrations of alcohol, ethanol, and at acidic pH | Citrobacter sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM: complete inhibition | Citrobacter sp. |  |
| EDTA | no inhibition | Citrobacter sp. | |
| F- | 1 mM: complete inhibition | Citrobacter sp. | |
| Hg2+ | 1 mM: complete inhibition | Citrobacter sp. | |
| iodoacetamide | no inhibition at 0.1 mM | Citrobacter sp. | |
| additional information | no inhibition by 1 mM EDTA and 2-mercaptoethanol; no inhibition by diethyldicarbonate, N-ethylmaleimide or iodoacetamide at 0.1 mM | Citrobacter sp. | |
| N-Benzoylphosphoric triamide | - |
Citrobacter sp. | |
| O,O-Dimethyl-O-(2,2-dichlorovinyl) phosphate | - |
Citrobacter sp. | |
| O,O-Dimethyl-O-(3-methyl-4-nitrophenyl) phosphate | - |
Citrobacter sp. | |
| phenylphosphorodiamide | - |
Citrobacter sp. | |
| phenylthiophosphorodiamide | - |
Citrobacter sp. | |
| Sodium laurylsulfate | 35 mM: complete inhibition | Citrobacter sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
urethane | - |
Citrobacter sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | 80% of activity in a cytosolic fraction | Citrobacter sp. | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metal ions not required | Citrobacter sp. |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | inactive at high concentrations of alcohol, 20% ethanol, 8.9% residual activity after 2 h at 37°C | Citrobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter sp. | - |
isolated from mouse feces | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Citrobacter sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | urethanase activity detected in the cells not in the culture medium | Citrobacter sp. | - |
| additional information | urethanase activity detected in the cells not in the culture medium | Citrobacter sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Citrobacter sp. |
| 0.254 | - |
- |
Citrobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetamide + H2O | 880% of the activity with ethylcarbamate | Citrobacter sp. | ? | - |
? | |
| benzamide + H2O | 680% of the activity with ethylcarbamate | Citrobacter sp. | ? | - |
? | |
| benzylcarbamate + H2O | 148% of the activity with ethylcarbamate | Citrobacter sp. | CO2 + NH3 + benzoate | - |
? | |
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Citrobacter sp. | CO2 + NH3 + ethanol | equimolar amounts of ammonia and ethanol | ? | |
| ethylcarbamate + H2O | urethane, potentially carcinogenic, mutagenic and teratogenic to human | Citrobacter sp. | CO2 + NH3 + ethanol | noncarcinogenic compounds | ? | |
| methylcarbamate + H2O | 64% of the activity with ethylcarbamate | Citrobacter sp. | CO2 + NH3 + methanol | - |
? | |
| additional information | substrate specificity | Citrobacter sp. | ? | - |
? | |
| additional information | no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea | Citrobacter sp. | ? | - |
? | |
| additional information | no activity with glycinamide, N-alkyl ureas, N-allylurea, ethyl esters of organic acids, ethyl acetate, ethyl benzoate, diethyl carbonate, phenylphosphorodiamide, phenylthiophosphorodiamide, N-benzoylphosphoric triamide | Citrobacter sp. | ? | - |
? | |
| n-butylcarbamate + H2O | 224% of the activity with ethylcarbamate | Citrobacter sp. | CO2 + NH3 + n-butanol | - |
? | |
| n-butyramide + H2O | 1000% of the activity with ethylcarbamate | Citrobacter sp. | ? | - |
? | |
| phenylcarbamate + H2O | 96% of the activity with ethylcarbamate | Citrobacter sp. | CO2 + NH3 + phenol | - |
? | |
| tert-butylcarbamate + H2O | 24.4% of the activity with ethylcarbamate | Citrobacter sp. | CO2 + NH3 + tert-butanol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Citrobacter sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
15 min, complete loss of activity | Citrobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Citrobacter sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
half of maximum activity at pH 5.0 | Citrobacter sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | does not require any cofactor | Citrobacter sp. |
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Release 2023.1 (January 2023)
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