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Literature summary for 3.5.1.70 extracted from
- Cloning and sequencing of the aculeacin A acylase-encoding gene from Actinoplanes utahensis and expression in Streptomyces lividans (1992), Gene, 119, 29-35.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | useful in producing peptide nuclei, i.e. deacetylated cyclic hexapeptides, for creating new antifungal agents by introducing different acyl moieties | Actinoplanes utahensis |
| synthesis | useful in producing peptide nuclei for creating new antifungal agents by introducing different acyl moieties | Actinoplanes utahensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Streptomyces lividans JT46 | Actinoplanes utahensis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Actinoplanes utahensis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19100 | - |
1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence | Actinoplanes utahensis |
| 60300 | - |
1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence | Actinoplanes utahensis |
| 84070 | - |
precursor peptide of aculeacin A acylase, calculated from nucleotide sequence | Actinoplanes utahensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinoplanes utahensis | - |
NRRL 12052 | - |
| Actinoplanes utahensis NRRL 12052 | - |
NRRL 12052 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | enzyme is translated as single precursor polypeptide and then processed by proteolytic modification to the active form consisting of two subunits | Actinoplanes utahensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from recombinant strain Streptomyces lividans JT46 | Actinoplanes utahensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | from recombinant strain Streptomyces lividans, about 80% detected in culture filtrate | Actinoplanes utahensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.1 | - |
from recombinant strain Streptomyces lividans JT46 | Actinoplanes utahensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aculeacin A + H2O | - |
Actinoplanes utahensis | cyclo-hexapeptide + palmitic acid | - |
? |  |
| aculeacin A + H2O | - |
Actinoplanes utahensis NRRL 12052 | cyclo-hexapeptide + palmitic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence | Actinoplanes utahensis |
| More | enzyme is translated as single precursor polypeptide and then processed by proteolytic processing to the active form consisting of two subunits | Actinoplanes utahensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAC | - |
Actinoplanes utahensis |
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