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Literature summary for 3.5.1.7 extracted from
- Enzymatic synthesis and breakdown of a pyrimidine, orotic acid. III. ureidosuccinase (1955), J. Biol. Chem., 212, 909-920.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | - |
Faecalicatena orotica |  |
| glutathione | effect less than half that of cysteine | Faecalicatena orotica | |
| sulfhydryl compound | - |
Faecalicatena orotica | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Sodium thioglycolate | - |
Faecalicatena orotica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Faecalicatena orotica | |
| Fe2+ | - |
Faecalicatena orotica | |
| Mn2+ | absolute requirement | Faecalicatena orotica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ureidosuccinic acid + H2O | Faecalicatena orotica | only the L-isomer is attacked, the D form is completely inactive | ? | - |
? | |
| additional information | Faecalicatena orotica | L-carboxymethylhydantoin is no substrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Faecalicatena orotica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Faecalicatena orotica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Faecalicatena orotica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C stable for at least 10 days if kept in vacuo, loses activity rapidly when exposed to air either at 0°C or -16°C | Faecalicatena orotica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoylglutamate + H2O | small amount of activity found | Faecalicatena orotica | ? | - |
? | |
| L-carboxymethylhydantoin + H2O | inert substrate | Faecalicatena orotica | CO2 + ? | - |
? | |
| L-ureidosuccinic acid + H2O | only the L-isomer was attacked, the D form is completely inactive | Faecalicatena orotica | L-aspartic acid + CO2 + NH3 | - |
? | |
| L-ureidosuccinic acid + H2O | only the L-isomer is attacked, the D form is completely inactive | Faecalicatena orotica | ? | - |
? | |
| additional information | L-carboxymethylhydantoin is no substrate | Faecalicatena orotica | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.5 | - |
Faecalicatena orotica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | 9.6 | - |
Faecalicatena orotica |
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Release 2023.1 (January 2023)
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