Enzymes of vitamin B6 degradation. Purification and properties of two N-acetylamidohydrolases
Huynh, M.S.; Snell, E.E.; J. Biol. Chem. 260, 2379-2383 (1985) 
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
Dicarboxylic acids
competitive
Arthrobacter sp.
diphosphate
competitive
Arthrobacter sp.
Hg2+
0.01 mM, 15 min, 82% activity remains
Arthrobacter sp.
Itaconic acid
competitive, most effective inhibitor, Ki: 0.74 mM
Arthrobacter sp.
malonate
competitive, Ki: 20 mM
Arthrobacter sp.
additional information
no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids
Arthrobacter sp.
phosphate
competitive
Arthrobacter sp.
sulfite
noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition
Arthrobacter sp.
Tricarboxylic acids
competitive
Arthrobacter sp.
Zn2+
1 mM: 40% inhibition
Arthrobacter sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic data
Arthrobacter sp.
0.025
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 25°C, Tris-HCl, pH 7.0
Arthrobacter sp.
0.123
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0
Arthrobacter sp.
0.25
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, Tris-HCl, pH 8.0
Arthrobacter sp.
0.56
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5
Arthrobacter sp.
0.68
-
3-(acetamidomethylene)succinate
compound A, 25°C, pH 7.0
Arthrobacter sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
some inorganic cations activate
Arthrobacter sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36600
-
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
205000
-
Cr-7, gel filtration
Arthrobacter sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
involved in pyridoxine degradation
?
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp. IA
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
additional information
Pseudomonas sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
additional information
Arthrobacter sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
additional information
Pseudomonas sp. IA
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arthrobacter sp.
-
Cr-7
-
Pseudomonas sp.
-
IA
-
Pseudomonas sp. IA
-
IA
-
Purification (Commentary)
Commentary
Organism
Cr-7
Arthrobacter sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Arthrobacter sp.
3.79
-
25°C
Arthrobacter sp.
Storage Stability
Storage Stability
Organism
Cr-7, -20°C, 0.1 M Tris-HCl, pH 7.0, stable for several months
Arthrobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Pseudomonas sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Arthrobacter sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Pseudomonas sp. IA
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
?
-
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
?
-
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
involved in pyridoxine degradation
172028
Arthrobacter sp.
?
-
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
?
-
-
-
-
3-(acetamidomethylene)succinate + H2O
compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate
172028
Arthrobacter sp.
acetate + 4-oxobutanoate + NH3 + CO2
-
172028
Arthrobacter sp.
?
additional information
high substrate specificity
172028
Arthrobacter sp.
?
-
-
-
-
additional information
enzyme highly specific, inducible amidohydrolase
172028
Arthrobacter sp.
?
-
-
-
-
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp.
?
-
-
-
-
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Arthrobacter sp.
?
-
-
-
-
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp. IA
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
multimer
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Arthrobacter sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
pH 8.0, 1 min, stable at
Arthrobacter sp.
100
-
1 min, 65% activity retained
Arthrobacter sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
turnover no./subunit, 25°C
Arthrobacter sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 4.2, effect of buffer on pH optimum
Arthrobacter sp.
6
7
-
Arthrobacter sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
5
rapid loss of activity
Arthrobacter sp.
6
10
stable at room temperature
Arthrobacter sp.
8
-
70°C, 1 min, stable at
Arthrobacter sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.74
-
Itaconic acid
competitive, most effective inhibitor
Arthrobacter sp.
20
-
malonate
competitive
Arthrobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Dicarboxylic acids
competitive
Arthrobacter sp.
diphosphate
competitive
Arthrobacter sp.
Hg2+
0.01 mM, 15 min, 82% activity remains
Arthrobacter sp.
Itaconic acid
competitive, most effective inhibitor, Ki: 0.74 mM
Arthrobacter sp.
malonate
competitive, Ki: 20 mM
Arthrobacter sp.
additional information
no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids
Arthrobacter sp.
phosphate
competitive
Arthrobacter sp.
sulfite
noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition
Arthrobacter sp.
Tricarboxylic acids
competitive
Arthrobacter sp.
Zn2+
1 mM: 40% inhibition
Arthrobacter sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.74
-
Itaconic acid
competitive, most effective inhibitor
Arthrobacter sp.
20
-
malonate
competitive
Arthrobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic data
Arthrobacter sp.
0.025
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 25°C, Tris-HCl, pH 7.0
Arthrobacter sp.
0.123
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0
Arthrobacter sp.
0.25
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, Tris-HCl, pH 8.0
Arthrobacter sp.
0.56
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5
Arthrobacter sp.
0.68
-
3-(acetamidomethylene)succinate
compound A, 25°C, pH 7.0
Arthrobacter sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
some inorganic cations activate
Arthrobacter sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36600
-
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
205000
-
Cr-7, gel filtration
Arthrobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
involved in pyridoxine degradation
?
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp. IA
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
additional information
Pseudomonas sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
additional information
Arthrobacter sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
additional information
Pseudomonas sp. IA
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
Cr-7
Arthrobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Arthrobacter sp.
3.79
-
25°C
Arthrobacter sp.
Storage Stability (protein specific)
Storage Stability
Organism
Cr-7, -20°C, 0.1 M Tris-HCl, pH 7.0, stable for several months
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Pseudomonas sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Arthrobacter sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Pseudomonas sp. IA
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
?
-
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
?
-
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
involved in pyridoxine degradation
172028
Arthrobacter sp.
?
-
-
-
-
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
?
-
-
-
-
3-(acetamidomethylene)succinate + H2O
compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate
172028
Arthrobacter sp.
acetate + 4-oxobutanoate + NH3 + CO2
-
172028
Arthrobacter sp.
?
additional information
high substrate specificity
172028
Arthrobacter sp.
?
-
-
-
-
additional information
enzyme highly specific, inducible amidohydrolase
172028
Arthrobacter sp.
?
-
-
-
-
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp.
?
-
-
-
-
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Arthrobacter sp.
?
-
-
-
-
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp. IA
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
multimer
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Arthrobacter sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
pH 8.0, 1 min, stable at
Arthrobacter sp.
100
-
1 min, 65% activity retained
Arthrobacter sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
turnover no./subunit, 25°C
Arthrobacter sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 4.2, effect of buffer on pH optimum
Arthrobacter sp.
6
7
-
Arthrobacter sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
5
rapid loss of activity
Arthrobacter sp.
6
10
stable at room temperature
Arthrobacter sp.
8
-
70°C, 1 min, stable at
Arthrobacter sp.
Other publictions for EC 3.5.1.66
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
172028
Huynh
Enzymes of vitamin B6 degradat ...
Arthrobacter sp., Pseudomonas sp., Pseudomonas sp. IA
J. Biol. Chem.
260
2379-2383
1985
-
-
-
-
-
-
10
6
-
1
2
7
-
6
-
-
1
-
-
-
2
1
13
1
1
-
2
1
2
-
3
-
2
-
-
-
-
-
-
-
-
-
-
10
2
6
-
1
2
7
-
-
-
1
-
-
2
1
13
1
1
-
2
1
2
-
3
-
-
-
-
-
-
-