Data extracted from this reference:
Inhibitors
Dicarboxylic acids
competitive
Arthrobacter sp.
diphosphate
competitive
Arthrobacter sp.
Hg2+
0.01 mM, 15 min, 82% activity remains
Arthrobacter sp.
Itaconic acid
competitive, most effective inhibitor, Ki: 0.74 mM
Arthrobacter sp.
malonate
competitive, Ki: 20 mM
Arthrobacter sp.
additional information
no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids
Arthrobacter sp.
phosphate
competitive
Arthrobacter sp.
sulfite
noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition
Arthrobacter sp.
Tricarboxylic acids
competitive
Arthrobacter sp.
Zn2+
1 mM: 40% inhibition
Arthrobacter sp.
KM Value [mM]
additional information
additional information
kinetic data
Arthrobacter sp.
0.025
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 25°C, Tris-HCl, pH 7.0
Arthrobacter sp.
0.123
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0
Arthrobacter sp.
0.25
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, Tris-HCl, pH 8.0
Arthrobacter sp.
0.56
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5
Arthrobacter sp.
0.68
3-(acetamidomethylene)succinate
compound A, 25°C, pH 7.0
Arthrobacter sp.
Metals/Ions
additional information
some inorganic cations activate
Arthrobacter sp.
Molecular Weight [Da]
36600
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
205000
Cr-7, gel filtration
Arthrobacter sp.
Natural Substrates/ Products (Substrates)
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
involved in pyridoxine degradation
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp. IA
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
additional information
Pseudomonas sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
additional information
Arthrobacter sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
additional information
Pseudomonas sp. IA
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
Organism
Purification (Commentary)
Specific Activity [micromol/min/mg]
additional information
Arthrobacter sp.
3.79
25°C
Arthrobacter sp.
Storage Stability
Cr-7, -20°C, 0.1 M Tris-HCl, pH 7.0, stable for several months
Arthrobacter sp.
Substrates and Products (Substrate)
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
172028
Pseudomonas sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
172028
Arthrobacter sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
172028
Pseudomonas sp. IA
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
involved in pyridoxine degradation
172028
Arthrobacter sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
?
3-(acetamidomethylene)succinate + H2O
compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate
172028
Arthrobacter sp.
acetate + 4-oxobutanoate + NH3 + CO2
172028
Arthrobacter sp.
?
additional information
high substrate specificity
172028
Arthrobacter sp.
?
additional information
enzyme highly specific, inducible amidohydrolase
172028
Arthrobacter sp.
?
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp.
?
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Arthrobacter sp.
?
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp. IA
?
Subunits
multimer
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C]
25
assay at
Arthrobacter sp.
Temperature Stability [°C]
70
pH 8.0, 1 min, stable at
Arthrobacter sp.
100
1 min, 65% activity retained
Arthrobacter sp.
Turnover Number [1/s]
2.3
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
turnover no./subunit, 25°C
Arthrobacter sp.
pH Optimum
additional information
pI: 4.2, effect of buffer on pH optimum
Arthrobacter sp.
pH Stability
4
5
rapid loss of activity
Arthrobacter sp.
6
10
stable at room temperature
Arthrobacter sp.
8
70°C, 1 min, stable at
Arthrobacter sp.
Ki Value [mM]
0.74
Itaconic acid
competitive, most effective inhibitor
Arthrobacter sp.
20
malonate
competitive
Arthrobacter sp.
Inhibitors (protein specific)
Dicarboxylic acids
competitive
Arthrobacter sp.
diphosphate
competitive
Arthrobacter sp.
Hg2+
0.01 mM, 15 min, 82% activity remains
Arthrobacter sp.
Itaconic acid
competitive, most effective inhibitor, Ki: 0.74 mM
Arthrobacter sp.
malonate
competitive, Ki: 20 mM
Arthrobacter sp.
additional information
no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids
Arthrobacter sp.
phosphate
competitive
Arthrobacter sp.
sulfite
noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition
Arthrobacter sp.
Tricarboxylic acids
competitive
Arthrobacter sp.
Zn2+
1 mM: 40% inhibition
Arthrobacter sp.
Ki Value [mM] (protein specific)
0.74
Itaconic acid
competitive, most effective inhibitor
Arthrobacter sp.
20
malonate
competitive
Arthrobacter sp.
KM Value [mM] (protein specific)
additional information
additional information
kinetic data
Arthrobacter sp.
0.025
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 25°C, Tris-HCl, pH 7.0
Arthrobacter sp.
0.123
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0
Arthrobacter sp.
0.25
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, Tris-HCl, pH 8.0
Arthrobacter sp.
0.56
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5
Arthrobacter sp.
0.68
3-(acetamidomethylene)succinate
compound A, 25°C, pH 7.0
Arthrobacter sp.
Metals/Ions (protein specific)
additional information
some inorganic cations activate
Arthrobacter sp.
Molecular Weight [Da] (protein specific)
36600
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
205000
Cr-7, gel filtration
Arthrobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
involved in pyridoxine degradation
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp. IA
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
additional information
Pseudomonas sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
additional information
Arthrobacter sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
additional information
Pseudomonas sp. IA
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
Purification (Commentary) (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
additional information
Arthrobacter sp.
3.79
25°C
Arthrobacter sp.
Storage Stability (protein specific)
Cr-7, -20°C, 0.1 M Tris-HCl, pH 7.0, stable for several months
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
172028
Pseudomonas sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
172028
Arthrobacter sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
172028
Pseudomonas sp. IA
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
involved in pyridoxine degradation
172028
Arthrobacter sp.
?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
?
3-(acetamidomethylene)succinate + H2O
compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate
172028
Arthrobacter sp.
acetate + 4-oxobutanoate + NH3 + CO2
172028
Arthrobacter sp.
?
additional information
high substrate specificity
172028
Arthrobacter sp.
?
additional information
enzyme highly specific, inducible amidohydrolase
172028
Arthrobacter sp.
?
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp.
?
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Arthrobacter sp.
?
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp. IA
?
Subunits (protein specific)
multimer
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C] (protein specific)
25
assay at
Arthrobacter sp.
Temperature Stability [°C] (protein specific)
70
pH 8.0, 1 min, stable at
Arthrobacter sp.
100
1 min, 65% activity retained
Arthrobacter sp.
Turnover Number [1/s] (protein specific)
2.3
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
turnover no./subunit, 25°C
Arthrobacter sp.
pH Optimum (protein specific)
additional information
pI: 4.2, effect of buffer on pH optimum
Arthrobacter sp.
pH Stability (protein specific)
4
5
rapid loss of activity
Arthrobacter sp.
6
10
stable at room temperature
Arthrobacter sp.
8
70°C, 1 min, stable at
Arthrobacter sp.
Other publictions for EC
172028
Huynh
Enzymes of vitamin B6 degradat ...
Arthrobacter sp., Pseudomonas sp., Pseudomonas sp. IA
J. Biol. Chem.
260
2379-2383
1985
-
-
-
-
-
-
10
6
-
1
2
7
-
6
-
-
1
-
-
-
2
1
13
1
-
1
-
2
1
2
-
3
-
2
-
-
-
-
-
-
-
-
-
-
10
2
6
-
1
2
7
-
-
-
1
-
-
2
1
13
1
1
-
2
1
2
-
3
-
-
-
-
-
-
-