Literature summary for 3.5.1.65 extracted from

  • Soda, K.; Uchiyama, K.; Ogata, K.
    Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part II. Purification and properties of the enzyme hydrolyzing L-theanine, D-theanine and the related compounds (1966), Agric. Biol. Chem., 30, 547-557.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 10 mM: strong inhibition Pseudomonas aeruginosa
EDTA 10 mM: 30% inhibition Pseudomonas aeruginosa
HgCl2 1 mM: strong inhibition Pseudomonas aeruginosa
iodoacetate 2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol Pseudomonas aeruginosa
additional information no inhibition by urea, p-chloromercuribenzoate, NaF or KF Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.8
-
L-Theanine partial purified enzyme Pseudomonas aeruginosa
9.2
-
D-theanine partial purified enzyme Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation by phosphate ions Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N5-ethyl-L-glutamine + H2O Pseudomonas aeruginosa
-
L-glutamate + ethylamine
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.83
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity Pseudomonas aeruginosa ?
-
?
additional information no activity with L-asparagine, D-asparagine or acetamide Pseudomonas aeruginosa ?
-
?
L-glutamine + H2O
-
Pseudomonas aeruginosa L-glutamate + NH3
-
?
D-glutamine + H2O
-
Pseudomonas aeruginosa D-glutamate + NH3
-
?
N5-ethyl-L-glutamine + H2O
-
Pseudomonas aeruginosa L-glutamate + ethylamine
-
?
N5-ethyl-D-glutamine + H2O D-theanine, probably by the same enzyme like L-theanine Pseudomonas aeruginosa D-glutamate + ethylamine
-
?
theanine + hydroxylamine transferase reaction Pseudomonas aeruginosa gamma-glutamylhydroxamic acid + ethylamine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
60 min, stable at over a wide range of pH Pseudomonas aeruginosa
55
-
above 55°C: rapid inactivation of enzyme Pseudomonas aeruginosa
60
-
15 min, rapid inactivation of enzyme Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas aeruginosa
8.5 9
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10.5 about half of maximum activity at pH 5.5 and 10.5 Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 quite stable between Pseudomonas aeruginosa